GCAT Protein (His tag)
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- Target See all GCAT Proteins
- GCAT (Glycine C-Acetyltransferase (GCAT))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GCAT protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human AKB ligase / GCAT (full length, N-term HIS tag) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product GCAT Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- GCAT (Glycine C-Acetyltransferase (GCAT))
- Alternative Name
- Akb Ligase,gcat (GCAT Products)
- Synonyms
- kbl Protein, ECK3607 Protein, JW3592 Protein, KBL Protein, AI526977 Protein, Kbl Protein, glycine C-acetyltransferase S homeolog Protein, glycine C-acetyltransferase Protein, 2-amino-3-ketobutyrate CoA ligase Protein, Glycine C-acetyltransferase Protein, glycine c-acetyltransferase Protein, glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) Protein, gcat.S Protein, gcat Protein, kbl Protein, Cagg_0385 Protein, Athe_2207 Protein, ABOO_RS03655 Protein, Mrub_1767 Protein, Mesil_2957 Protein, COB47_1985 Protein, Lbys_3455 Protein, Ftrac_1580 Protein, Pedsa_0139 Protein, GCAT Protein, Gcat Protein
- Background
- The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14.
- Molecular Weight
- 45.3 kDa
- NCBI Accession
- NP_055106
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