GCAT Protein (AA 22-419) (His tag)
-
- Target See all GCAT Proteins
- GCAT (Glycine C-Acetyltransferase (GCAT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-419
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GCAT protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MSALAQLRGI LEGELEGIRG AGTWKSERVI TSRQGPHIRV DGVSGGILNF CANNYLGLSS HPEVIQAGLQ ALEEFGAGLS SVRFICGTQS IHKNLEAKIA RFHQREDAIL YPSCYDANAG LFEALLTPED AVLSDELNHA SIIDGIRLCK AHKYRYRHLD MADLEAKLQE AQKHRLRLVA TDGAFSMDGD IAPLQEICCL ASRYGALVFM DECHATGFLG PTGRGTDELL GVMDQVTIIN STLGKALGGA SGGYTTGPGP LVSLLRQRAR PYLFSNSLPP AVVGCASKAL DLLMGSNTIV QSMAAKTQRF RSKMEAAGFT ISGASHPICP VMLGDARLAS RMADDMLKRG IFVIGFSYPV VPKGKARIRV QISAVHSEED IDRCVEAFVE VGRLHGALP
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product GCAT Protein
-
Glycine C-Acetyltransferase (GCAT) (AA 1-419) protein (GST tag)
GCAT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glycine C-Acetyltransferase (GCAT) (AA 22-419) protein (His tag)Crystallography grade GCAT Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glycine C-Acetyltransferase (GCAT) (AA 19-416) protein (His tag)Crystallography grade GCAT Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Denatured
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.4M urea, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- Target
- GCAT (Glycine C-Acetyltransferase (GCAT))
- Alternative Name
- GCAT (GCAT Products)
- Synonyms
- kbl Protein, ECK3607 Protein, JW3592 Protein, KBL Protein, AI526977 Protein, Kbl Protein, glycine C-acetyltransferase S homeolog Protein, glycine C-acetyltransferase Protein, 2-amino-3-ketobutyrate CoA ligase Protein, Glycine C-acetyltransferase Protein, glycine c-acetyltransferase Protein, glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) Protein, gcat.S Protein, gcat Protein, kbl Protein, Cagg_0385 Protein, Athe_2207 Protein, ABOO_RS03655 Protein, Mrub_1767 Protein, Mesil_2957 Protein, COB47_1985 Protein, Lbys_3455 Protein, Ftrac_1580 Protein, Pedsa_0139 Protein, GCAT Protein, Gcat Protein
- Background
- The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Recombinant human GCAT protein, fused to His-tag at N-terminus, was expressed in E.coli.
- Molecular Weight
- 45.0kDa (419aa)
- NCBI Accession
- NP_055106
- UniProt
- O75600
-
