ATP5F1 Protein (His tag)

Catalog No. ABIN2715048

Product Details

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ATP5F1 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human ATP5F1 (full length, N-term HIS tag) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Alternative Name: Atp5f1 (ATP5F1 Products)

Synonyms: PIG47 Protein, C76477 Protein, wu:fb59g11 Protein, wu:fj08b08 Protein, zgc:101887 Protein, ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1 Protein, ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1 S homeolog Protein, ATP synthase F(0) complex subunit B1, mitochondrial Protein, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 Protein, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1 Protein, ATP5F1 Protein, atp5f1.S Protein, atp5f1 Protein, LOC479901 Protein, Atp5f1 Protein

Background: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel.

Molecular Weight: 28.9 kDa

NCBI Accession: NP_001679

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process