MYT1 Protein (AA 1-1127) (Strep Tag)

Catalog No. ABIN3136430

Product Details

Target: MYT1 (Myelin Transcription Factor 1 (MYT1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1127

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This MYT1 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MSSESDDKRA RTRSKTLRGP PETTGADLSC PTPGCTGSGH VRGKYSRHRS LQSCPLAKKR KLEDAETEHL VSKRKSHPLR LALDEGYRMD SDGSEDAEVK DVSVSDESEG PLEEAEAEMS GQEEIHHPQT AEGKSLIKPH FDSNPTSSPS GFSKSSYSSY QGIIATSLLN LGQIAEEALV KEDSVSVAKL SPTVVHQLQD EAAMGVNSDE GEKDLFIQPE DVEEVIEVTS ERSQEPCPQS LKDMVSEESS KQKGVLGHEE EGEEEEEDEE EEDEEEEEEG EEGEEEEEEE EEEEEEEDEE EEEEEEEAAP NVIFGEDTSH TSVQKASPEF RGPELSSPKP EYSVIVEVRS DDDKDEDSRS QKSAVTDESE MYDMMTRGNL GLLEQAIALK AEQVRAVCES GCPPAEQGHL GPGEPGKMAK PLDVVRKSCY SKDPSRVEKR EIKCPTPGCD GTGHVTGLYP HHRSLSGCPH KDRIPPEILA MHENVLKCPT PGCTGQGHVN SNRNTHRSLS GCPIAAAEKL AKSHEKQQLQ TGDPPKNNSN SDRILRPMCF VKQLEVPPYG SYRPNVAPAT PRANLAKELE KFSKVTFDYA SFDAQVFGKR MLAPKIQTSE TSPKAFQCFD YSHDAEAAHM AATAILNLST RCWEMPENLS TKPQDLPSKA VDIEVDENGT LDLSMHKHRK RENTFPSSSS CSSSPGVKSP DVSQRQSSTS APSSSMTSPQ SSQASRQDEW DRPLDYTKPS RLREEEPEES EPAAHSFASS EADDQEVSEE NFEERKYPGE VTLTNFKLKF LSKDIKKELL TCPTPGCDGS GHITGNYASH RSLSGCPLAD KSLRNLMAAH SADLKCPTPG CDGSGHITGN YASHRSLSGC PRAKKSGLKV APTKDDKEDP ELMKCPVPGC VGLGHISGKY ASHRSASGCP LAARRQKEGA LNGSSFSWKS LKNEGPTCPT PGCDGSGHAN GSFLTHRSLS GCPRATFAGK KGKLSGDEIL SPKFKTSDVL ENDEEIKQLN QEIRDLNESN SEMEAAMVQL QSQISSMEKN LKNIEEENKL IEEQNEALFL ELSGLSQALI QSLANIRLPH MEPICEQNFD AYVNTLTDMY SNQDCYQNPE NKGLLETIKQ AVRGIQV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: MYT1 (Myelin Transcription Factor 1 (MYT1))

Alternative Name: Myt1 (MYT1 Products)

Synonyms: fc56h05 Protein, myt1 Protein, si:dkey-202m22.5 Protein, wu:fc56h05 Protein, C20orf36 Protein, MTF1 Protein, MYTI Protein, PLPB1 Protein, ZC2HC4A Protein, Nzf2 Protein, Nzf2a Protein, Nzf2b Protein, Nztf2 Protein, myelin transcription factor 1 Protein, myelin transcription factor 1a Protein, myelin transcription factor 1 S homeolog Protein, MYT1 Protein, myt1a Protein, Myt1 Protein, myt1.S Protein

Background: Myelin transcription factor 1 (MyT1) (Neural zinc finger factor 2) (NZF-2),FUNCTION: Binds to the promoter region of genes encoding proteolipid proteins of the central nervous system. May play a role in the development of neurons and oligodendroglia in the CNS. May regulate a critical transition point in oligodendrocyte lineage development by modulating oligodendrocyte progenitor proliferation relative to terminal differentiation and up-regulation of myelin gene transcription (By similarity). {ECO:0000250, ECO:0000269|PubMed:9373037}.

Molecular Weight: 123.6 kDa

UniProt: Q8CFC2

Pathways: Cell Division Cycle