Gc (AA 17-474) protein (His tag)
Origin: Human
Host: Human Cells
Recombinant
Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.
ELISA, WB, SDS, MS
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- Target
- Gc
- Protein Type
- Recombinant
- Protein Characteristics
- AA 17-474
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- His tag
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Mass Spectrometry (MS)
- Purpose
- Recombinant Human GC Protein is produced by our Mammalian expression system and the target gene encoding Leu17-Leu474 is expressed with a 6His tag at the C-terminus.
- Sequence
- LERGRDYEKN KVCKEFSHLG KEDFTSLSLV LYSRKFPSGT FEQVSQLVKE VVSLTEACCA EGADPDCYDT RTSALSAKSC ESNSPFPVHP GTAECCTKEG LERKLCMAAL KHQPQEFPTY VEPTNDEICE AFRKDPKEYA NQFMWEYSTN YGQAPLSLLV SYTKSYLSMV GSCCTSASPT VCFLKERLQL KHLSLLTTLS NRVCSQYAAY GEKKSRLSNL IKLAQKVPTA DLEDVLPLAE DITNILSKCC ESASEDCMAK ELPEHTVKLC DNLSTKNSKF EDCCQEKTAM DVFVCTYFMP AAQLPELPDV ELPTNKDVCD PGNTKVMDKY TFELSRRTHL PEVFLSKVLE PTLKSLGECC DVEDSTTCFN AKGPLLKKEL SSFIDKGQEL CADYSENTFT EYKKKLAERL KAKLPDATPT ELAKLVNKRS DFASNCCSIN SPPLYCDSEI DAELKNILVD HHHHHH
- Purity
- Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
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Gc (AA 1-474) protein (GST tag)
Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- Gc
- Synonyms
- GLP1 Protein, GLP2 Protein, GRPP Protein, GLP-1 Protein, glucagon Protein, GCG Protein, Gcg Protein
- Target Type
- Viral Protein
- Gene ID
- 2638
- UniProt
- P02774
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