Gc (AA 17-474) protein (His tag)
Origin: Human
Host: Human Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- Target
- Gc
- Protein Type
- Recombinant
- Protein Characteristics
- AA 17-474
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- His tag
- Purpose
- Recombinant Human Vitamin D-Binding Protein/VDB/Gc-globulin (C-6His)
- Sequence
- LERGRDYEKN KVCKEFSHLG KEDFTSLSLV LYSRKFPSGT FEQVSQLVKE VVSLTEACCA EGADPDCYDT RTSALSAKSC ESNSPFPVHP GTAECCTKEG LERKLCMAAL KHQPQEFPTY VEPTNDEICE AFRKDPKEYA NQFMWEYSTN YGQAPLSLLV SYTKSYLSMV GSCCTSASPT VCFLKERLQL KHLSLLTTLS NRVCSQYAAY GEKKSRLSNL IKLAQKVPTA DLEDVLPLAE DITNILSKCC ESASEDCMAK ELPEHTVKLC DNLSTKNSKF EDCCQEKTAM DVFVCTYFMP AAQLPELPDV ELPTNKDVCD PGNTKVMDKY TFELSRRTHL PEVFLSKVLE PTLKSLGECC DVEDSTTCFN AKGPLLKKEL SSFIDKGQEL CADYSENTFT EYKKKLAERL KAKLPDATPT ELAKLVNKRS DFASNCCSIN SPPLYCDSEI DAELKNILVD HHHHHH
- Characteristics
- Recombinant Human Vitamin D-Binding Protein/GC is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Leu17-Leu474) of Human GC fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Gc (AA 1-474) protein (GST tag)
Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.2.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
-
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- Gc
- Synonyms
- GLP1 Protein, GLP2 Protein, GRPP Protein, GLP-1 Protein, glucagon Protein, GCG Protein, Gcg Protein
- Sub Type
- Fusionprotein
- Target Type
- Viral Protein
- Background
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Vitamin D-Binding Protein (DBP) is a member of the ALB/AFP/VDB family. DBP is a secreted protein and contains three albumin domains. The primary structure contains 28 cysteine residues forming multiple disulfide bonds. DBP acts as a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. DBP binds to vitamin D and its plasma metabolites and transports them to target tissues. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
Alternative Names: Vitamin D-Binding Protein, DBP, VDB, Gc-Globulin, Group-Specific Component, GC - Molecular Weight
- 52.3 kDa
- UniProt
- P02774
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