UBE2I Protein (AA 1-158) (GST tag)

Catalog No. ABIN7313902

Product Details

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Protein Type: Recombinant

Protein Characteristics: AA 1-158

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2I protein is labelled with GST tag.

Application: SDS-PAGE (SDS), ELISA, Western Blotting (WB), Mass Spectrometry (MS)

Purpose: Recombinant Human UBE2I Protein is produced by our E. coli expression system and the target gene encoding Met1-Ser158 is expressed with a GST tag at the N-terminus.

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMSG IALSRLAQER KAWRKDHPFG FVAVPTKNPD GTMNLMNWEC AIPGKKGTPW EGGLFKLRML FKDDYPSSPP KCKFEPPLFH PNVYPSGTVC LSILEEDKDW RPAITIKQIL LGIQELLNEP NIQDPAQAEA YTIYCQNRVE YEKRVRAQAK KFAPS

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as a 0.2 μM filtered solution of 50 mM HEPES, 150 mM NaCl, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Alternative Name: UBE2I (UBE2I Products)

Synonyms: C358B7.1 Protein, P18 Protein, UBC9 Protein, ubc9 Protein, ube2ia Protein, zUbc9 Protein, 5830467E05Rik Protein, F830028O17Rik Protein, Ubce2i Protein, Ubce9 Protein, UbcE2A Protein, ubce9 Protein, T13J8.70 Protein, T13J8_70 Protein, UBIQUITIN-PROTEIN LIGASE Protein, ubiquitin conjugating enzyme 9 Protein, ubiquitin conjugating enzyme E2 I Protein, ubiquitin-conjugating enzyme E2Ib Protein, ubiquitin-conjugating enzyme E2L Protein, ubiquitin-conjugating enzyme E2I Protein, ubiquitin conjugating enzyme E2I S homeolog Protein, ubiquitin conjugating enzyme 9 Protein, SUMO-conjugating enzyme UBC9 Protein, UBE2I Protein, ube2ib Protein, UBE2L Protein, Ube2i Protein, ube2i.S Protein, UBC9 Protein, ubc-9 Protein, LOC108703134 Protein

Gene ID: 7329

UniProt: P63279

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway