CA8 Protein (AA 2-290) (His tag)
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- Target See all CA8 Proteins
- CA8 (Carbonic Anhydrase VIII (CA8))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-290
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CA8 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Mass Spectrometry (MS)
- Purpose
- Recombinant Human CA8 Protein is produced by our E.coli expression system and the target gene encoding Ala2-Gln290 is expressed with a 6His tag at the C-terminus.
- Sequence
- MADLSFIEDT VAFPEKEEDE EEEEEGVEWG YEEGVEWGLV FPDANGEYQS PINLNSREAR YDPSLLDVRL SPNYVVCRDC EVTNDGHTIQ VILKSKSVLS GGPLPQGHEF ELYEVRFHWG RENQRGSEHT VNFKAFPMEL HLIHWNSTLF GSIDEAVGKP HGIAIIALFV QIGKEHVGLK AVTEILQDIQ YKGKSKTIPC FNPNTLLPDP LLRDYWVYEG SLTIPPCSEG VTWILFRYPL TISQLQIEEF RRLRTHVKGA ELVEGCDGIL GDNFRPTQPL SDRVIRAAFQ LEHHHHHH
- Purity
- Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
- Top Product
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 500 mM NaCl, 1 mM DTT, pH 8.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- CA8 (Carbonic Anhydrase VIII (CA8))
- Alternative Name
- CA8 (CA8 Products)
- Synonyms
- AW546993 Protein, Ca8 Protein, Cals Protein, Cals1 Protein, Carp Protein, wdl Protein, zgc:110118 Protein, CA-VIII Protein, CALS Protein, CAMRQ3 Protein, CARP Protein, carbonic anhydrase 8 Protein, carbonic anhydrase VIII Protein, CAH8 Protein, Car8 Protein, ca8 Protein, CA8 Protein
- Gene ID
- 767
- UniProt
- P35219
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