UBE2A Protein (AA 1-152) (GST tag,His tag)

Catalog No. ABIN7313882

Product Details

Target: UBE2A (ube2a) (Ubiquitin-Conjugating Enzyme E2A (ube2a))

Protein Type: Recombinant

Protein Characteristics: AA 1-152

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2A protein is labelled with GST tag,His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Mass Spectrometry (MS)

Purpose: Recombinant Human UBE2A Protein is produced by our E.coli expression system and the target gene encoding Met1-Cys152 is expressed with a GST, 6His tag at the N-terminus.

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGDDDDKSPM GSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR DC

Purity: Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as a 0.2 μM filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10 % Glycerol, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: UBE2A (ube2a) (Ubiquitin-Conjugating Enzyme E2A (ube2a))

Alternative Name: UBE2A (ube2a Products)

Synonyms: BHR6A Protein, HR6A Protein, hhr6a Protein, rad6a Protein, ubc2 Protein, mp:zf637-2-000771 Protein, si:bz46j2.4 Protein, wu:fa01h11 Protein, HHR6A Protein, MRXS30 Protein, MRXSN Protein, RAD6A Protein, UBC2 Protein, Mhr6a Protein, ubiquitin conjugating enzyme E2 A Protein, ubiquitin conjugating enzyme E2 A L homeolog Protein, ubiquitin-conjugating enzyme E2A (RAD6 homolog) Protein, ubiquitin-conjugating enzyme E2A Protein, UBE2A Protein, ube2a.L Protein, ube2a Protein, Ube2a Protein

Gene ID: 7319

UniProt: P49459