DCK Protein (AA 1-260) (His tag)
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- Target See all DCK Proteins
- DCK (Deoxycytidine Kinase (DCK))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-260
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This DCK protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- DCK, 1-260aa, Human, His tag, E.coli
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product DCK Protein
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Deoxycytidine Kinase (DCK) (AA 1-260) protein (His tag)
DCK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Deoxycytidine Kinase (DCK) (AA 1-260) protein (GST tag)DCK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Deoxycytidine Kinase (DCK) (AA 161-260) protein (GST tag)DCK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Deoxycytidine Kinase (DCK) (AA 1-260) protein (Strep Tag)Crystallography grade DCK Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Deoxycytidine Kinase (DCK) (AA 1-260) protein (Strep Tag)Crystallography grade DCK Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Deoxycytidine Kinase (DCK) protein (DYKDDDDK Tag)DCK Origin: Human Host: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Deoxycytidine Kinase (DCK) protein (Myc-DYKDDDDK Tag)DCK Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 1 mM DTT, 0.1 mM PMSF, 2mM EDTA, 10% Glycerol
- Storage
- 4 °C
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- Target
- DCK (Deoxycytidine Kinase (DCK))
- Alternative Name
- DCK (DCK Products)
- Synonyms
- dck Protein, DCK Protein, wu:fc15f06 Protein, zgc:101771 Protein, dck1 Protein, deoxycytidine kinase Protein, deoxycytidine kinase, gene 2 L homeolog Protein, deoxycytidine kinase, gene 2 Protein, Deoxycytidine kinase Protein, deoxycytidine kinase, gene 1 L homeolog Protein, DCK Protein, dck Protein, dck.2.L Protein, dck.2 Protein, Dck Protein, FPV059 Protein, FPV151 Protein, dck.1.L Protein
- Background
- DCK is a key enzyme in the salvage of deoxyribonucleosides and in the activation of clinically relevant nucleoside analogues. This protein is responsible for the 5`-phosphorylation of purine and pyrimidine deoxynucleosides to the corresponding monophosphates using ATP or UTP as phosphate donors. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. Recombinant human DCK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: MGC117410, MGC138632, Deoxycytidine kinase. NCBI no.: NP_000779
- Molecular Weight
- 34.6 kDa(296aa), confirmed by MALDI-TOF
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