DCK Protein (AA 1-260) (His tag,T7 tag)

Catalog No. ABIN1691941

This Recombinant DCK protein is produced in Escherichia coli (E. coli).

Quick Overview for DCK Protein (AA 1-260) (His tag,T7 tag) (ABIN1691941)

Target: DCK (Deoxycytidine Kinase (DCK))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-260

Purification tag / Conjugate: This DCK protein is labelled with His tag,T7 tag.

Purpose: Recombinant Human Deoxycytidine Kinase/DCK (N-6His, T7 tag)

Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSMATPPK RSCPSFSASS EGTRIKKISI EGNIAAGKST FVNILKQLCE DWEVVPEPVA RWCNVQSTQD EFEELTMSQK NGGNVLQMMY EKPERWSFTF QTYACLSRIR AQLASLNGKL KDAEKPVLFF ERSVYSDRYI FASNLYESEC MNETEWTIYQ DWHDWMNNQF GQSLELDGII YLQATPETCL HRIYLRGRNE EQGIPLEYLE KLHYKHESWL LHRTLKTNFD YLQEVPILTL DVNEDFKDKY ESLVEKVKEF LSTL

Characteristics: Recombinant Human Deoxycytidine Kinase/DCK is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Leu260) of Human DCK fused with a 6His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: DCK (Deoxycytidine Kinase (DCK))

Alternative Name: DCK

Sub Type: Fusionprotein

Background: Deoxycytidine Kinase (DCK) is a member of the DCK/DGK family. DCK exists as a homodimer and is localized to the nucleus. DCK is required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG), and deoxyadenosine (dA). DCK has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. In addition, DCK is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. DCK is clinically important because of its relationship to drug resistance and sensitivity.
Alternative Names: Deoxycytidine Kinase, dCK, DCK

Molecular Weight: 34 kDa

UniProt: P27707