STRO-1 antibody
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- Target
- STRO-1
- Reactivity
- Human, Rabbit, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Flow Cytometry (FACS), Immunocytochemistry (ICC)
- Specificity
- The mouse monoclonal antibody STRO-1 recognizes an extracellular epitope of the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.
- Cross-Reactivity (Details)
- Human
- Purification
- Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
- Purity
- > 95 % (by SDS-PAGE)
- Immunogen
- Human CD34 positive bone marrow cells
- Clone
- STRO-1
- Isotype
- IgM lambda
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- Application Notes
- Flow cytometry: Recommended dilution: 1-5 μg/mL.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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Immunohistochemical localization of the STRO-1 antigen in developing rat teeth by light microscopy and electron microscopy." in: Journal of electron microscopy, Vol. 58, Issue 6, pp. 363-73, (2009) (PubMed).
: "Recovery of stem cells from cryopreserved periodontal ligament." in: Journal of dental research, Vol. 84, Issue 10, pp. 907-12, (2005) (PubMed).
: "Homing of in vitro expanded Stro-1- or Stro-1+ human mesenchymal stem cells into the NOD/SCID mouse and their role in supporting human CD34 cell engraftment." in: Blood, Vol. 103, Issue 9, pp. 3313-9, (2004) (PubMed).
: "Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow." in: Journal of cell science, Vol. 116, Issue Pt 9, pp. 1827-35, (2003) (PubMed).
: "Number and proliferative capacity of osteogenic stem cells are maintained during aging and in patients with osteoporosis." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 16, Issue 6, pp. 1120-9, (2001) (PubMed).
: "Isolation and characterization of human clonogenic osteoblast progenitors immunoselected from fetal bone marrow stroma using STRO-1 monoclonal antibody." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 14, Issue 3, pp. 351-61, (1999) (PubMed).
: "Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1." in: Blood, Vol. 78, Issue 1, pp. 55-62, (1991) (PubMed).
: "
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Immunohistochemical localization of the STRO-1 antigen in developing rat teeth by light microscopy and electron microscopy." in: Journal of electron microscopy, Vol. 58, Issue 6, pp. 363-73, (2009) (PubMed).
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- Target
- STRO-1
- Background
- STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10 % of mononuclear cells, greater than 95 % of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.,STRO1
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