The monoclonal antibody will detect Podoplanin on the surface of lymphatic endothelial cells and some epithelial cell types by immunostaining or immunohistochemistry. The antibody does not cross-react with Human Podoplanin.
Immunohistochemistry (use diluted 1-10 μg/mL IgG). Works well with paraffin sections. Western blot (use 1-10 μg/mL IgG, will detect a 36-40 kDa band). Immunoprecipitation (use 1-5 μg IgG per 1 mLlysate or reaction volume). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.4, without preservatives or stabilizers
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Prior to reconstitution store at 2-8 °C. After reconstitution store the antibody (in aliquots) at -20 °C.
Uhrin, Zaujec, Breuss, Olcaydu, Chrenek, Stockinger, Fuertbauer, Moser, Haiko, Fässler, Alitalo, Binder, Kerjaschki: "Novel function for blood platelets and podoplanin in developmental separation of blood and lymphatic circulation." in: Blood, Vol. 115, Issue 19, pp. 3997-4005, (2010) (PubMed).
DAmico, Jones, Nye, Sapienza, Ramjuan, Reynolds, Robinson, Kostourou, Martinez, Aubyn, Grose, Thomas, Spencer-Dene, Zicha, Davies, Tybulewicz, Hodivala-Dilke: "Regulation of lymphatic-blood vessel separation by endothelial Rac1." in: Development (Cambridge, England), Vol. 136, Issue 23, pp. 4043-53, (2009) (PubMed).
NOYES, DOMM, WILLSLC: "Regulation of erythropoiesis. I. Erythropoietin assay as a clinical tool." in: Blood, Vol. 20, pp. 9-18 (PubMed).
Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a Mr 38,000 membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha