GABRB3 antibody (pSer408, pSer409)

Catalog No. ABIN6658226

Product Details anti-GABRB3 Antibody

Target: GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))

Binding Specificity: pSer408, pSer409

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRB3 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Anti-GABA(A) Receptor beta 3 pS408/pS409 Antibody is directed against rat GABA(A) Receptor beta 3. The antibody was affinity purified from monospecific antiserum by immunoaffintiy purification. Immunolabeling of the GABAA band is completely blocked by the phospho-peptide used as antigen but not by the corresponding dephospho-peptide. Reactivity is expected from the following species based on 100% sequence homology: bovine, canine, chicken, human, mouse, non-human primates, Xenopus and zebra fish.

Immunogen:

Immunogen: Anti-GABA(A) Receptor beta 3 pS408/pS409 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser408/409.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-GABA(A) Receptor beta 3 pS408/pS409 (Rabbit) antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 53 kDa in size corresponding to GABA(A) receptor beta 3 subunit phosphorylated at Ser408/409 in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

References for anti-GABRB3 antibody (ABIN6658226)

Pribiag, Stellwagen: "TNF-? downregulates inhibitory neurotransmission through protein phosphatase 1-dependent trafficking of GABA(A) receptors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 40, pp. 15879-93, (2013) (PubMed).

Target Details for GABRB3

Target: GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))

Alternative Name: GABA(A) Receptor beta 3 (GABRB3 Products)

Synonyms: GABRB3 antibody, eca5 antibody, LOC100230475 antibody, ECA5 antibody, A230092K12Rik antibody, AW049585 antibody, Cp1 antibody, Gabrb-3 antibody, beta3 antibody, gamma-aminobutyric acid type A receptor beta3 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, beta 3 antibody, gamma-aminobutyric acid type A receptor beta 3 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, subunit beta 3 antibody, GABRB3 antibody, gabrb3 antibody, Gabrb3 antibody

Background:

Synonyms: Gamma-aminobutyric acid receptor subunit beta-3, GABA(A) receptor subunit beta-3, Gabrb3, GABA(A) receptor subunit ß-3

Background: Anti-GABA(A) Receptor beta 3 pS408/pS409 Antibody detects GABA(A) Receptor beta 3. Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma- subunit is required for benzodiazepine modulation. Phosphorylation of serine 408 and serine 409 within the beta3 subunit have been shown to be critical for the functional modulation of beta3 containing recombinant receptors. GABA(A) Receptor beta 3 pS408/pS409 antibody is ideal for investigators involved in Neuroscience.

Gene Name: GABRB3

Gene ID: 24922

UniProt: P63079

Pathways: Sensory Perception of Sound