SMAD4 antibody (Thr277)

Catalog No. ABIN542927

Product Details anti-SMAD4 Antibody

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Binding Specificity: Thr277

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of SMAD4.

Cross-Reactivity: Human

Immunogen: A synthetic peptide (conjugated with KLH) corresponding to residues surrounding T277 of human SMAD4.

Application Details

Application Notes: Dot Blot (1:500)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-SMAD4 antibody (ABIN542927)

Zhang, Lui: "Transforming growth factor-?3 regulates cell junction restructuring via MAPK-mediated mRNA destabilization and Smad-dependent protein degradation of junctional adhesion molecule B (JAM-B)." in: Biochimica et biophysica acta, Vol. 1849, Issue 6, pp. 601-11, (2015) (PubMed).

Xiao, Ho, Liong, Nanji, Leung, Lau, Fung, Tipoe: "Epigallocatechin gallate attenuates fibrosis, oxidative stress, and inflammation in non-alcoholic fatty liver disease rat model through TGF/SMAD, PI3 K/Akt/FoxO1, and NF-kappa B pathways." in: European journal of nutrition, Vol. 53, Issue 1, pp. 187-99, (2014) (PubMed).

Xiao, Xing, Huo, Fung, Liong, Ching, Xu, Chang, So, Tipoe: "Lycium barbarum polysaccharides therapeutically improve hepatic functions in non-alcoholic steatohepatitis rats and cellular steatosis model." in: Scientific reports, Vol. 4, pp. 5587, (2014) (PubMed).

Wang, Lui: "Transforming growth factor-?1 attenuates junctional adhesion molecule-A and contributes to breast cancer cell invasion." in: European journal of cancer (Oxford, England : 1990), Vol. 48, Issue 18, pp. 3475-87, (2012) (PubMed).

Horvath, Henshall, Kench, Turner, Golovsky, Brenner, ONeill, Kooner, Stricker, Grygiel, Sutherland: "Loss of BMP2, Smad8, and Smad4 expression in prostate cancer progression." in: The Prostate, Vol. 59, Issue 3, pp. 234-42, (2004) (PubMed).

Sekiya, Oda, Matsuura, Akiyama: "Transcriptional regulation of the TGF-beta pseudoreceptor BAMBI by TGF-beta signaling." in: Biochemical and biophysical research communications, Vol. 320, Issue 3, pp. 680-4, (2004) (PubMed).

Li, Xin, Xu, Huang, Zhang, Chen, Zhang, Fu, Chang: "CHIP mediates degradation of Smad proteins and potentially regulates Smad-induced transcription." in: Molecular and cellular biology, Vol. 24, Issue 2, pp. 856-64, (2003) (PubMed).

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4 (SMAD4 Products)

Synonyms: smad4 antibody, DPC4 antibody, JIP antibody, MADH4 antibody, MYHRS antibody, AW743858 antibody, D18Wsu70e antibody, Madh4 antibody, XSmad4alpha antibody, Xsmad4 antibody, dpc4 antibody, jip antibody, madh4 antibody, xsmad4a antibody, SMAD family member 4 antibody, Smad4 protein antibody, SMAD family member 4, gene 1 L homeolog antibody, SMAD4 antibody, smad4 antibody, Smad4 antibody, smad4.1.L antibody

Gene ID: 4089

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy