CD19 antibody
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- Target See all CD19 Antibodies
- CD19 (CD19 Molecule (CD19))
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Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This CD19 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
- Specificity
- The rat monoclonal antibody 1D3 detects an extracellular epitope of murine CD19, 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells, it is lost on plasma cells.
- Cross-Reactivity (Details)
- Mouse
- Purification
- Purified by protein-G affinity chromatography.
- Purity
- > 95 % (by SDS-PAGE)
- Endotoxin Level
- Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
- Immunogen
- Mouse CD19-transfected cell line
- Clone
- 1D3
- Isotype
- IgG2a
- Top Product
- Discover our top product CD19 Primary Antibody
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- Application Notes
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Functional application: This antibody can induce down-regulation of CD19, affecting the proportions of B cell subpopulations.
Flow cytometry: Recommended dilution: 1-4 μg/mL - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4
- Preservative
- Azide free
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 8, pp. 4062-72, (2003) (PubMed).
: "Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement." in: Blood, Vol. 99, Issue 2, pp. 584-9, (2002) (PubMed).
: "The role of the CD19/CD21 complex in B cell processing and presentation of complement-tagged antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 1, pp. 163-72, (2001) (PubMed).
: "Self-renewal of B-1 lymphocytes is dependent on CD19." in: European journal of immunology, Vol. 26, Issue 1, pp. 238-42, (1996) (PubMed).
: "The signaling activity of murine CD19 is regulated during cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 1, pp. 48-56, (1996) (PubMed).
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The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 8, pp. 4062-72, (2003) (PubMed).
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- Target
- CD19 (CD19 Molecule (CD19))
- Alternative Name
- CD19 (CD19 Products)
- Synonyms
- B4 antibody, CVID3 antibody, AW495831 antibody, CD19 antibody, CD19 molecule antibody, CD19 antigen antibody, CD19 antibody, Cd19 antibody
- Background
- CD19 Molecule,CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.,B4, Leu-12, Cvid
- Gene ID
- 12478
- UniProt
- P25918
- Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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