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anti-Human BDNF antibody for Enzyme Immunoassay

Recommended BDNF Antibody (supplied by: Log in to see )

Antigen
Brain-Derived Neurotrophic Factor (BDNF) Antibodies
  • ANON2
  • bdnf
  • Bdnf
  • BDNF
  • bdnf-A
  • BULN2
Reactivity
Human
268
99
78
9
8
6
6
5
2
2
1
1
1
Host
Rabbit
220
80
9
8
7
5
Clonality
Polyclonal
Conjugate
This BDNF antibody is conjugated to Biotin
30
21
14
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
254
137
89
47
34
32
31
17
14
11
3
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN115983
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008808967 ABIN115985 EIA Func IHC (fro) WB Rabbit Log in to see Polyclonal 3
0.0008808967 ABIN115984 EIA WB Biotin Rabbit Log in to see Polyclonal 1
0.0008808967 ABIN115986 EIA Func IHC (fro) WB Rabbit Log in to see Polyclonal 3

Top referenced anti-BDNF antibody for Enzyme Immunoassay

  • Human Polyclonal BDNF Primary Antibody for EIA, WB - ABIN115983 (1 Publications): Hartog, Dittrich, Pieneman, Jansen, Frankl-Vilches, Lessmann, Lilliehook, Goldman, Gahr: Brain-derived neurotrophic factor signaling in the HVC is required for testosterone-induced song of female canaries. in The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 (PubMed)

  • Human Polyclonal BDNF Primary Antibody for EIA, Func - ABIN115985 (3 Publications): Miknyoczki, Wan, Chang, Dobrzanski, Ruggeri, Dionne, Buchkovich: The neurotrophin-trk receptor axes are critical for the growth and progression of human prostatic carcinoma and pancreatic ductal adenocarcinoma xenografts in nude mice. in Clinical cancer research : an official journal of the American Association for Cancer Research 2002 (PubMed)

  • Human Polyclonal BDNF Primary Antibody for EIA, Func - ABIN115986 (3 Publications): Wirth, Patz, Wahle: Transcellular induction of neuropeptide Y expression by NT4 and BDNF. in Proceedings of the National Academy of Sciences of the United States of America 2005 (PubMed)

Similar anti-BDNF Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 2 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 99 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 32 Antibodies
Functional Studies (Func) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 25 Antibodies
Immunofluorescence (IF) 31 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 84 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 37 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Neutralization (Neut) 3 Antibodies

General

Antigen Brain-Derived Neurotrophic Factor (BDNF) Antibodies
Reactivity Human
(268), (99), (78), (9), (8), (6), (6), (5), (2), (2), (1), (1), (1)
Host Rabbit
(220), (80), (9), (8), (7), (5)
Clonality
Polyclonal
Conjugate This BDNF antibody is conjugated to Biotin
(30), (21), (14), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(254), (137), (89), (47), (34), (32), (31), (17), (14), (11), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BDNF Antibody

Target Details BDNF Application Details Handling References for anti-BDNF Antibody (ABIN115983) Images
Specificity Recognizes Human BDNF.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Affinity chromatography
Immunogen Highly purified (>98%) recombinant Human BDNF

Target Details BDNF

Product Details anti-BDNF Antibody Application Details Handling References for anti-BDNF Antibody (ABIN115983) Images back to top
Antigen
Alternative Name BDNF (BDNF Antibody Abstract)
Background Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. All neurotrophins have six conserved cysteine residues and share a 55 % sequence identity at the amino acid level. BDNF is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non neuronal cells. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients. In addition, functional studies showed that age-associated changes in BDNF-mediated pathways can enhance inflammation and increase myocardial injury after myocardial infarction in the aging heart.Synonyms: Abrineurin, Brain-derived neurotrophic factor
Gene ID 627
NCBI Accession NP_001137277
UniProt P23560
Research Area Cardiovascular, Atherosclerosis, Alzheimer's Disease, Hormones
Pathways RTK Signaling

Application Details

Product Details anti-BDNF Antibody Target Details BDNF Handling References for anti-BDNF Antibody (ABIN115983) Images back to top
Application Notes ELISA: Direct: To detect Human BDNF by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Human BDNF. Sandwich: To detect Human BDNF by Sandwich ELISA (using 100 μL/well antibody solution)a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Anti-Human BDNF (PP1000P1 or PP1000P1) as a captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human BDNF. Western blot: To detect Human BDNF by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents
Restrictions For Research Use only

Handling

Product Details anti-BDNF Antibody Target Details BDNF Application Details References for anti-BDNF Antibody (ABIN115983) Images back to top
Reconstitution Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-BDNF Antibody (ABIN115983)

Product Details anti-BDNF Antibody Target Details BDNF Application Details Handling Images back to top
Product cited in:

Hartog, Dittrich, Pieneman, Jansen, Frankl-Vilches, Lessmann, Lilliehook, Goldman, Gahr: "Brain-derived neurotrophic factor signaling in the HVC is required for testosterone-induced song of female canaries." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 49, pp. 15511-9, 2009