BDNF antibody (Internal Region)

Catalog No. ABIN5539690

The Goat Polyclonal anti-BDNF antibody (ABIN5539690) specifically detects BDNF in ELISA and IF. The antibody is reactive with Human samples.

Quick Overview for BDNF antibody (Internal Region) (ABIN5539690)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BDNF antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-BDNF Antibody

Binding Specificity: Internal Region

Purpose: BDNF

Sequence: ETKCNPMGYT KE

Specificity: This antibody is expected to recognise all reported isoforms (NP_001700.2, NP_001700.2, NP_733928.1, NP_733929.1, NP_733930.1, NP_733931.1)

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2000.

Comment:

Immunofluorescence: Expression of the protein seen in the nucleus and cytoplasm of Glioblastoma U251 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF

Background: BDNF , brain-derived neurotrophic factor, MGC34632, neurotrophin

Gene ID: 627

NCBI Accession: NP_001700, NP_733928, NP_733929, NP_733930, NP_733931

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity