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anti-Human BDNF antibody for Blocking Antibody

Recommended BDNF Antibody (supplied by: Log in to see )

Antigen
Brain-Derived Neurotrophic Factor (BDNF) Antibodies
  • ANON2
  • bdnf
  • Bdnf
  • BDNF
  • bdnf-A
  • BULN2
Reactivity
Human
263
98
77
9
7
6
6
5
2
1
1
1
1
Host
Rabbit
218
78
8
8
8
5
Clonality
Polyclonal
Conjugate
This BDNF antibody is un-conjugated
31
21
14
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
249
133
88
46
32
32
30
14
14
11
4
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1001932
$ 374.22
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008700791 ABIN636731 Inhibition ELISA WB Rabbit Log in to see Polyclonal

Similar anti-BDNF Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 2 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 98 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 32 Antibodies
Functional Studies (Func) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 25 Antibodies
Immunofluorescence (IF) 31 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 85 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 38 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Neutralization (Neut) 3 Antibodies

General

Antigen Brain-Derived Neurotrophic Factor (BDNF) Antibodies
Reactivity Human
(263), (98), (77), (9), (7), (6), (6), (5), (2), (1), (1), (1), (1)
Host Rabbit
(218), (78), (8), (8), (8), (5)
Clonality
Polyclonal
Conjugate This BDNF antibody is un-conjugated
(31), (21), (14), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
(249), (133), (88), (46), (32), (32), (30), (14), (14), (11), (4), (3), (2), (1), (1), (1), (1), (1)
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Product Details anti-BDNF Antibody

Target Details BDNF Application Details Handling Images
Purification Antigen affinity purified polyclol antibody
Sterility Sterile filtered
Immunogen recombint hBDNF (human Brain-Derived Neurotrophic Factor)
Isotype IgG

Target Details BDNF

Product Details anti-BDNF Antibody Application Details Handling Images back to top
Antigen
Alternative Name Brain Derived Neurotrophic Factor (BDNF Antibody Abstract)
Background Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBDNF (human Brain-Derived Neurotrophic Factor). Anti-Human BDNF specific antibody was purified by affinity chromatography employing immobilized hBDNF matrix.
Pathways RTK Signaling

Application Details

Product Details anti-BDNF Antibody Target Details BDNF Handling Images back to top
Application Notes To yield one-half maximal inhibition [ND₅₀] of the biological activity of hBDNF (2.0 µg/µl), a concentration of 0.54 - 0.81 µg/µl of this antibody is required. To detect hBDNF by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBDNF. To detect hBDNF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Human BDNF (PK-AB815-66212B) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBDNF. To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunihostochemistry: NA. To detect hBDNF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Human BDNF (PK-AB815-66212B) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBDNF. To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-BDNF Antibody Target Details BDNF Application Details Images back to top
Format Lyophilized
Concentration To yield one-half maximal inhibition [ND₅₀] of the biological activity of hBDNF (2.0 µg/µl), a concentration of 0.54 - 0.81 µg/µl of this antibody is required.
Buffer A sterile-filtered antibody solution was lyophilized from PBS, pH 7.2.
Handling Advice The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. Centrifuge vial prior to opening. Reconstitute in sterile water at a concentration of 0.1-1.0 mg/ml.
Storage -20 °C
Expiry Date 24 months