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anti-Mouse (Murine) BDNF antibody for Immunohistochemistry (Frozen Sections)

Recommended BDNF Antibody (supplied by: Log in to see )

Antigen
Brain-Derived Neurotrophic Factor (BDNF) Antibodies
  • ANON2
  • bdnf
  • Bdnf
  • BDNF
  • bdnf-A
  • BULN2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
213
75
73
9
7
6
6
5
2
1
1
1
Host
Rabbit
214
32
8
8
8
5
Clonality
Polyclonal
Conjugate
This BDNF antibody is un-conjugated
29
20
12
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
206
133
65
23
14
12
12
11
9
6
3
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN115985
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088051456 ABIN1858130 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 139-249 Log in to see Polyclonal
0.00088051456 ABIN2617538 IHC (fro) IHC (p) Chicken IgY Log in to see Polyclonal
0.00088051456 ABIN409162 ICC IHC (fro) IF IHC (p) IHC WB Rabbit Log in to see Polyclonal
0.00088051456 ABIN409229 ICC IHC (fro) IF IHC WB Rabbit IgG Log in to see Polyclonal
0.00088051456 ABIN2962022 IHC (fro) IP IHC (p) WB Chicken IgY Log in to see Polyclonal

Top referenced anti-BDNF antibody for Immunohistochemistry (Frozen Sections)

  • Human Polyclonal BDNF Primary Antibody for EIA, Func - ABIN115985 (3 Publications): Hartog, Dittrich, Pieneman, Jansen, Frankl-Vilches, Lessmann, Lilliehook, Goldman, Gahr: Brain-derived neurotrophic factor signaling in the HVC is required for testosterone-induced song of female canaries. in The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 (PubMed)

Similar anti-BDNF Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 26 Antibodies 15 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 1 Antibodies
Functional Studies (Func) 2 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 3 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 22 Antibodies 24 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies 12 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 56 Antibodies

General

Antigen Brain-Derived Neurotrophic Factor (BDNF) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(213), (75), (73), (9), (7), (6), (6), (5), (2), (1), (1), (1)
Host Rabbit
(214), (32), (8), (8), (8), (5)
Clonality
Polyclonal
Conjugate This BDNF antibody is un-conjugated
(29), (20), (12), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(206), (133), (65), (23), (14), (12), (12), (11), (9), (6), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-BDNF Antibody

Target Details BDNF Application Details Handling References for anti-BDNF Antibody (ABIN115985) Images
Specificity The antibody recognises BDNF.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse and Rat.
Purification Affinity Chromatography employing immobilized Human BDNF matrix
Immunogen Highly purified (>98%) E.coli derived recombinant Human BDNF (Cat.-No PA047)

Target Details BDNF

Product Details anti-BDNF Antibody Application Details Handling References for anti-BDNF Antibody (ABIN115985) Images back to top
Antigen
Alternative Name BDNF (BDNF Antibody Abstract)
Background Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. All neurotrophins have six conserved cysteine residues and share a 55 % sequence identity at the amino acid level. BDNF is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non neuronal cells. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients. In addition, functional studies showed that age-associated changes in BDNF-mediated pathways can enhance inflammation and increase myocardial injury after myocardial infarction in the aging heart.Synonyms: Abrineurin, Brain-derived neurotrophic factor
Gene ID 627
NCBI Accession NP_001137277
UniProt P23560
Research Area Cardiovascular, Atherosclerosis, Alzheimer's Disease, Hormones
Pathways RTK Signaling

Application Details

Product Details anti-BDNF Antibody Target Details BDNF Handling References for anti-BDNF Antibody (ABIN115985) Images back to top
Application Notes ELISA: Indirect To detect hBDNF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hBDNF. Sandwich To detect hBDNF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith a biotinylated anti-Human BDNF (PP1000B1 / PP1000B2) as a detection antibody,allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBDNF. Western blot: To detect hBDNF by Western blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. The detection limit for recombinant hBDNF is 1.5 - 3.0 ng/lane, under eitherreducing or non-reducing conditions.
Protocol NEUROTROPHIN IMMUNOHISTOCHEMISTRYThe neurotrophins have proved difficult to localize which may be due to masking by, forexample, their association with the trk receptors or very low concentrations. Wheresuccess has been achieved, the conditions required vary greatly for different tissues. Aprotocol has been included for central nervous system tissue and, while it is similar tocommonly used methods, it is important to give strict attention to details such as thoroughwashing, fixative and detergent concentrations, concentration and quality of the primaryantibodies and length of incubations, etc. You may find it possible to use alternateprotocols, however we have experienced many failures using variations of the currentprotocol (and some failures when strict adherence to the procedure is maintained). Asuitable procedure to stain nerve terminals is still being developed. Neurotrophinreceptors have proved much easier to localize. It is recommended that you include a few sections of adult rat cerebellum, spinal cord orkidney in each experiment since these are tissues which are the easiest to stain. Protocol for Immunohistochemistry in the Central Nervous System(At all steps thorough washing is necessary to reduce background)Fixation: Animals are perfused with 1% sodium nitrite in phosphate buffered saline (PBS) (about 50ml) followed by 1 liter of Zamboni's fixative (4% formaldehyde, 15% picric acid in 0. 1Mphosphate buffer). Post fix for no longer than 2 hours. Tissue Preparation: Tissues are removed and washed briefly with PBS followed by cryoprotection in 30%sucrose in PBS overnight at 4°C. 30 µm cryostat sections are cut and washed with agitationin: PBS (1x15min)50% ethanol (3x15min)
Restrictions For Research Use only

Handling

Product Details anti-BDNF Antibody Target Details BDNF Application Details References for anti-BDNF Antibody (ABIN115985) Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2, without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-BDNF Antibody (ABIN115985)

Product Details anti-BDNF Antibody Target Details BDNF Application Details Handling Images back to top
Product cited in:

Hartog, Dittrich, Pieneman, Jansen, Frankl-Vilches, Lessmann, Lilliehook, Goldman, Gahr: "Brain-derived neurotrophic factor signaling in the HVC is required for testosterone-induced song of female canaries." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 49, pp. 15511-9, 2009

Background publications

Wirth, Patz, Wahle: "Transcellular induction of neuropeptide Y expression by NT4 and BDNF." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 8, pp. 3064-9, 2005

Miknyoczki, Wan, Chang, Dobrzanski, Ruggeri, Dionne, Buchkovich: "The neurotrophin-trk receptor axes are critical for the growth and progression of human prostatic carcinoma and pancreatic ductal adenocarcinoma xenografts in nude mice." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 8, Issue 6, pp. 1924-31, 2002