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Mouse (Murine) CSF1 ELISA Kit

Recommended CSF1 Product (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA Kits
  • CSF1
  • csf1-1
  • zgc:172186
  • CSF-1
  • MCSF
  • C87615
  • Csfm
  • op
  • colony stimulating factor 1 (macrophage)
  • colony stimulating factor 1a (macrophage)
  • macrophage colony stimulating factor alpha
  • CSF1
  • csf1a
  • Csf1
  • MCSF ALPHA
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
24
19
5
2
2
2
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
1-350 pg/mL
Minimum Detection Limit
1 pg/mL
Application
ELISA
Options
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Catalog No. ABIN625417
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
12.876543 ABIN415526 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 1.37-1000 pg/mL 1.37 pg/mL Log in to see
12.876543 ABIN3198466 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 31.25-2000 pg/mL 31.25 pg/mL Log in to see
12.876543 ABIN1379958 Sandwich ELISA Cell Lysate, Serum, Plasma 32-1000 pg/mL 32 pg/mL Log in to see
12.876543 ABIN2708571 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
12.876543 ABIN2113596 Cell Culture Supernatant, Tissue Homogenate, Serum, Plasma (EDTA) Log in to see
1 ABIN3032826 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 1.4-350 pg/mL 1.4 pg/mL Log in to see
1 ABIN1884550 Sandwich ELISA Plasma, Serum, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN2683921 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Log in to see 3
1 ABIN1029181 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN1112745 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN2042584 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN2114493 Cell Lysate, Serum, Plasma 32-1000 pg/mL 32 pg/mL Log in to see
1 ABIN4947463 Sandwich ELISA Log in to see
1 ABIN1846993 Serum, Plasma, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN2543215 Sandwich ELISA Serum, Plasma, Biological Fluids 6.25-400 pg/mL 6.25 pg/mL Log in to see
1 ABIN2536289 Serum, Plasma, Biological Fluids 1-80 pg/mL 1 pg/mL Log in to see
1 ABIN2116217 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN455771 Log in to see
1 ABIN1720485 Log in to see

Top referenced CSF1 ELISA Kit for ELISA

  • Mouse (Murine) CSF1 ELISA Kit for Sandwich ELISA - ABIN625417 (1 Publications): Chitu, Gokhan, Gulinello, Branch, Patil, Basu, Stoddart, Mehler, Stanley: Phenotypic characterization of a Csf1r haploinsufficient mouse model of adult-onset leukodystrophy with axonal spheroids and pigmented glia (ALSP). in Neurobiology of disease 2015 (PubMed)

Similar CSF1 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 20 ELISA Kits

General

Antigen Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA Kits
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(24), (19), (5), (2), (2), (2), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 1-350 pg/mL
Minimum Detection Limit 1 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details CSF1 ELISA Kit

Target details Application Details Handling References for CSF1 Kit (ABIN625417) Images
Purpose Mouse M-CSF ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Serum, Plasma, Cell Culture Supernatant
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Sensitivity < 1 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details CSF1 ELISA Kit Application Details Handling References for CSF1 Kit (ABIN625417) Images back to top
Antigen
Alternative Name M-CSF / CSF1 (CSF1 ELISA Kit Abstract)
Gene ID 12977
UniProt P07141
Pathways RTK Signaling

Application Details

Product Details CSF1 ELISA Kit Target details Handling References for CSF1 Kit (ABIN625417) Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernatants and urine. Suggested dilution for normal serum/plasma: 2 fold*. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent B should be diluted 5-fold with deionized or distilled water before use.
4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture supernates) into Item C vial to prepare a 50 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 7 µL MCSF standard (50 ng/mL) from the vial of Item C, into a tube with 993 µL Assay Diluent A or 1x Assay Diluent B to prepare a 350 pg/mL standard solution. Pipette 300 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the 350 pg/mL standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 7 µL standard + 993 µL 200 µL 200 µL 200 µL 200 µL 200 µL 200myl 350 140 56.00 22.40 8.96 3.58 1.43 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 300-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 40 µL of HRP-Streptavidin concentrate into a tube with 12 ml 1x Assay Diluent B to prepare a 300-fold diluted HRP- Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A Mouse MCSF concentration (pg/mL) 1 10 100 1000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent B Mouse MCSF concentration (pg/mL) 1 10 100 1000 O D =4 50 n m 0.01 0.1 1 10
Sensitivity: The minimum detectable dose of MCSF is typically less than 1 pg/mL.
Recovery: Recovery was determined by spiking various levels mouse MCSF into mouse serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 98.81 93-106 Plasma 103.0 83-125 Cell culture media 133.9 127-138
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 105.4 103.7 109.8 Range ( %) 97-103 95-109 103-115 1:4 Average % of Expected 103.8 93.19 111.1 Range ( %) 95-111 82-107 105-117
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Assay Precision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Restrictions For Research Use only

Handling

Product Details CSF1 ELISA Kit Target details Application Details References for CSF1 Kit (ABIN625417) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

References for CSF1 Kit (ABIN625417)

Product Details CSF1 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Chitu, Gokhan, Gulinello, Branch, Patil, Basu, Stoddart, Mehler, Stanley: "Phenotypic characterization of a Csf1r haploinsufficient mouse model of adult-onset leukodystrophy with axonal spheroids and pigmented glia (ALSP)." in: Neurobiology of disease, Vol. 74, pp. 219-28, 2015

Images

Product Details CSF1 ELISA Kit Target details Application Details Handling References for CSF1 Kit (ABIN625417) back to top
Supplier Images
ELISA image for CSF1 ELISA Kit (Colony Stimulating Factor 1 (Macrophage)) (ABIN625417) Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA Kit