M-CSF/CSF1 ELISA Kit

Catalog No. ABIN6719805

Product Details M-CSF/CSF1 ELISA Kit

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Serum, Tissue Lysates

Analytical Method: Quantitative

Characteristics:

Synonyms: Colony stimulating factor 1, Colony stimulating factor 1 (macrophage), Colony stimulating factor macrophage specific, CSF 1, CSF-1, CSF1, CSF1_HUMAN, Csfm, Lanimostim, M CSF, M-CSF, Macrophage colony stimulating factor, Macrophage colony stimulating factor 1, MCSF, MGC41930, op, OTTHUMP00000013364, OTTHUMP00000013365, OTTMUSP00000032803, OTTMUSP00000032804, Processed macrophage colony-stimulating factor 1, RP11-195M16.2

Background: M-CSF, also called CSF1, consists of a 14-amino acid peptide, longer than the usual 8-to-11 mer recognized by most CTLs. The M-CSF gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb1. Although it is a single-copy gene, its expression results in the synthesis of several mRNAs, ranging in size from about 1.5 to 4.5 kb2. There are 2 forms of M-CSF, with 224 and 522 amino acids, resulting from alternative splicing3. Furthermore, M-CSF has a role in development of the placenta. Uterine M-CSF concentration is regulated by a synergistic action of estradiol and progesterone. M-CSF is produced by uterine glandular epithelial cells. It had been found that FMS, the M-CSF receptor, is expressed in placenta and choriocarcinoma cell lines4.

Gene Name: CCL3

Production: Natural and recombinant mouse M-CSF. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence:  K33-E262

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse M-CSF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, tissue lysates, serum or plasma (EDTA) to each empty well. It is recommended that each mouse M-CSF standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Sodium EDTA

ELISA Dilution: 15.6pg/mL-1000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for M-CSF/CSF1

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Alternative Name: M-CSF (CSF1 Products)

Synonyms: CSF-1 ELISA Kit, MCSF ELISA Kit, C87615 ELISA Kit, Csfm ELISA Kit, op ELISA Kit, csf1-1 ELISA Kit, zgc:172186 ELISA Kit, CSF1 ELISA Kit, csf1-2 ELISA Kit, zgc:158436 ELISA Kit, colony stimulating factor 1 ELISA Kit, colony stimulating factor 1 (macrophage) ELISA Kit, colony stimulating factor 1a (macrophage) ELISA Kit, macrophage colony-stimulating factor 1 ELISA Kit, macrophage colony stimulating factor ELISA Kit, colony stimulating factor 1b (macrophage) ELISA Kit, CSF1 ELISA Kit, Csf1 ELISA Kit, csf1a ELISA Kit, LOC396599 ELISA Kit, LOC100860895 ELISA Kit, csf1b ELISA Kit

Gene ID: 12977

NCBI Accession: NP_001107001

UniProt: P07141

Pathways: RTK Signaling