anti-Human CSF1R antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended CSF1R Antibody (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 1 Receptor (CSF1R) Antibodies
  • MGC89146
  • C-FMS
  • CD115
  • CSF-1R
  • CSFR
  • FIM2
  • FMS
  • HDLS
  • M-CSF-R
  • AI323359
  • Csfmr
  • Fim-2
  • Fms
  • M-CSFR
  • CSF-1-R
  • c-fms
  • mrfms
  • CSF1
  • M-CSF
  • csf1r
  • fms
  • panther
  • colony stimulating factor 1 receptor
  • csf1r protein
  • macrophage colony-stimulating factor receptor
  • colony stimulating factor 1 receptor, a
  • CSF1R
  • csf1r
  • m-csfr
  • Csf1r
  • csf1ra
Epitope
AA 694-744, pTyr723
40
38
21
20
19
19
15
14
12
11
10
9
9
8
7
6
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
343
201
84
22
12
9
8
6
4
3
3
3
2
2
2
1
Host
Rabbit
231
112
107
32
4
Clonality
Polyclonal
Conjugate
This CSF1R antibody is un-conjugated
34
28
25
18
18
13
11
9
9
8
8
7
6
6
5
5
5
5
5
5
4
3
3
2
2
2
2
1
1
1
1
1
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
307
227
197
155
83
57
28
16
15
12
8
7
6
5
4
3
3
3
3
2
2
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Catalog No. ABIN683788
$ 317.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2808113 IF (cc) IF (p) Alexa Fluor 594 Rabbit IgG AA 694-744, pTyr723 Log in to see Polyclonal
1 ABIN2811980 IF (p) Alexa Fluor 594 Rabbit IgG AA 890-940, pTyr923 Log in to see Polyclonal
1 ABIN683791 IF (cc) IF (p) Cy3 Rabbit IgG AA 694-744, pTyr723 Log in to see Polyclonal
1 ABIN683792 IF (cc) IF (p) Cy5 Rabbit IgG AA 694-744, pTyr723 Log in to see Polyclonal
1 ABIN683793 IF (cc) IF (p) Cy5.5 Rabbit IgG AA 694-744, pTyr723 Log in to see Polyclonal
1 ABIN683794 IF (cc) IF (p) Cy7 Rabbit IgG AA 694-744, pTyr723 Log in to see Polyclonal
1 ABIN683795 IF (cc) IF (p) FITC Rabbit IgG AA 694-744, pTyr723 Log in to see Polyclonal
1 ABIN880094 IF (cc) IF (p) Alexa Fluor 350 Rabbit IgG AA 694-744, pTyr723 Log in to see Polyclonal
1 ABIN880096 IF (cc) IF (p) Alexa Fluor 647 Rabbit IgG AA 694-744, pTyr723 Log in to see Polyclonal
1 ABIN880097 IF (cc) IF (p) Alexa Fluor 555 Rabbit IgG AA 694-744, pTyr723 Log in to see Polyclonal
1 ABIN880098 IF (cc) IF (p) Alexa Fluor 488 Rabbit IgG AA 694-744, pTyr723 Log in to see Polyclonal
1 ABIN5000596 IF (p) Alexa Fluor 680 Rabbit IgG AA 890-940, pTyr923 Log in to see Polyclonal
1 ABIN5000594 IF (cc) IF (p) Alexa Fluor 680 Rabbit IgG AA 694-744, pTyr723 Log in to see Polyclonal
1 ABIN5000597 IF (p) Alexa Fluor 750 Rabbit IgG AA 890-940, pTyr923 Log in to see Polyclonal
1 ABIN5000595 IF (cc) IF (p) Alexa Fluor 750 Rabbit IgG AA 694-744, pTyr723 Log in to see Polyclonal

General

Antigen Colony Stimulating Factor 1 Receptor (CSF1R) Antibodies
Epitope AA 694-744, pTyr723
(40), (38), (21), (20), (19), (19), (15), (14), (12), (11), (10), (9), (9), (8), (7), (6), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(343), (201), (84), (22), (12), (9), (8), (6), (4), (3), (3), (3), (2), (2), (2), (1)
Host Rabbit
(231), (112), (107), (32), (4)
Clonality
Polyclonal
Conjugate This CSF1R antibody is un-conjugated
(34), (28), (25), (18), (18), (13), (11), (9), (9), (8), (8), (7), (6), (6), (5), (5), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(307), (227), (197), (155), (83), (57), (28), (16), (15), (12), (8), (7), (6), (5), (4), (3), (3), (3), (3), (2), (2)
Pubmed 1 reference available
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Product Details anti-CSF1R Antibody

Target Details CSF1R Application Details Handling ProductDetails: References for anti-CSF1R antibody (ABIN683788) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human CSF1R around the phosphorylation site of tyrosine 723
Isotype IgG

Target Details CSF1R

Product Details anti-CSF1R Antibody Application Details Handling ProductDetails: References for anti-CSF1R antibody (ABIN683788) Images back to top
Antigen
Alternative Name Csf1r (CSF1R Antibody Abstract)
Background

This protein tyrosine kinase transmembrane receptor is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a member of the CSF1/PDGF receptor family of tyrosine protein kinases and contains 5 immunoglobulin like C2 type domains. CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Mutations in this gene have been associated with a predisposition to myeloid malignancy.

Subcellular location: Cytoplasm

Synonyms: FMS, CSFR, FIM2, HDLS, C-FMS, CD115, CSF-1R, M-CSF-R, Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, CSF-1-R, Proto-oncogene c-Fms, CSF1R

Gene ID 1436
UniProt P07333
Pathways RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization

Application Details

Product Details anti-CSF1R Antibody Target Details CSF1R Handling ProductDetails: References for anti-CSF1R antibody (ABIN683788) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-CSF1R Antibody Target Details CSF1R Application Details ProductDetails: References for anti-CSF1R antibody (ABIN683788) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

ProductDetails: References for anti-CSF1R antibody (ABIN683788)

Product Details anti-CSF1R Antibody Target Details CSF1R Application Details Handling Images back to top
Product cited in:

Król, Majchrzak, Mucha, Homa, Bulkowska, Jakubowska, Karwicka, Paw?owski, Motyl: "CSF-1R as an inhibitor of apoptosis and promoter of proliferation, migration and invasion of canine mammary cancer cells." in: BMC veterinary research, Vol. 9, pp. 65, 2013 Method employed by authors: Immunohistochemistry (IHC) Further details: After 30 min incubation in 5% bovine serum albumin (Sigma Aldrich) the rabbit polyclonal anti- p-CSF-1R (Tyr 723) antibodies diluted in 1% bovine serum were used, and then the slides were incubated at +4�C overnight.

Images

Product Details anti-CSF1R Antibody Target Details CSF1R Application Details Handling ProductDetails: References for anti-CSF1R antibody (ABIN683788) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Colony Stimulating Factor 1 Receptor (CSF1R) (AA 694-744), (pTyr723) antibody (ABIN683788) Staining of canine lymph node tissue with bs-3077r. The primary antibody bs-3077r was...
'Independent Validation' Badge
Antigen CSF1R (Colony Stimulating Factor 1 Receptor)
Lot Number 9I02V3
Method validated Immunohistochemistry
Positive Control canine lymph node tissue
Negative Control secondary antibody only
Notes The CSF1R antibody ABIN683788 specifically labels the targeted antigen in canine lymph node samples in IHC.
Primary Antibody ABIN683788
Secondary Antibody anti-rabbit (Vector, Vectastain ABC-Elite kit, PK 6101, lot 300712)
Protocol
  • Canine lymph node tissue is dissected and fixed in 4% buffered formalin ON at RT.
  • Deparaffinization and rehydration through graded xylene and graded alcohol series:
    • xylene twice for 3min.
    • 100% ethanol for 1min.
    • 95% ethanol for 1min.
    • 70% ethanol for 1min.
    • 50% ethanol for 1min.
    • H2O for 2min.
  • Boil the sections in the decloaking chamber (Biocare Medical):
    • fill the outer chamber with 500ml distilled water.
    • fill a plastic jar with diluted Antigen unmasking solution (Vector, H3301) diluted 1:100.
    • Boil sections for 8 minutes, turn it off and leave for 1h.
  • Rinse sections with distilled water 3 times.
  • Rinse sections with 0.05M Tris-HCl pH7.6, 3 times.
  • Block sections in normal goat serum (Vector, Vectastain ABC-Elite kit, PK 6101, lot 300712) diluted 1:50 for 1h at RT.
  • Blot excess serum from sections; do not rinse.
  • Incubate sections with primary antibody rabbit-anti CSF1R antibody (antibodies-online, ABIN683788, lot 9I02V3) diluted 1:100-1:500 in with 0.05M Tris-HCl, pH7.6 ON at 4°C. Include no primary antibody negative controls.
  • Rinse sections with 0.05M Tris-HCl pH7.6, 3 times. Keep negative controls in a separate jar.
  • Incubate sections with biotinylated secondary antibody anti-rabbit (Vector, Vectastain ABC-Elite kit, PK 6101, lot 300712) diluted 1:200 for 30min at RT.
  • Mix 20µl of reagent A and reagent B of the Vectastain Elite ABC HRP kit (Vectorlabs, PK-6101, lot 300712) in 1ml 0.05M TrisHCl pH7.6 and leave the mixture for 30min at RT.
  • Rinse sections with 0.05M Tris-HCl pH7.6, 3 times.
  • Incubate sections in the ABC reagent mix for 45min at RT.
  • Rinse sections with 0.05M Tris-HCl pH7.6 3 times.
  • DAB staining using peroxidase substrate kit (Vector, product SK-4100, lot 020612):
    • Mix thoroughly 2.5ml and 1 drop buffer stock.
    • Add 2 drops DAB and mix thoroughly.
    • Add 1 drop H2O2 and mix thoroughly.
  • Rinse sections with 0.05M Tris-HCl pH7.6 3 times.
  • Stain sections with Mayer HTX (Histolab, 01820, lot 00811.) for 2-3min at RT.
  • Rinse sections in tap water for 5-10min.
  • Dehydrate in fresh ethanol:
    • 95% ethanol twice for 2min.
    • 99.5% ethanol twice for 2min.
    • xylene twice for 2min.
  • Mount sections in Pertex mounting medium (Histolab, 00811).
  • Dry sections in a ventilated place.
  • Image acquisition (x10 and x20 objectives).
    Experimental Notes The blocking step with avidin and biotin was omitted for the no primary antibody negative controls. As a result, there is some unspecific staining in these sections. ABIN683788 worked nicely on the canine lymph node that was used as a positive control.
    Validation Images
    Immunohistochemistry validation image for anti-Colony Stimulating Factor 1 Receptor (CSF1R) (AA 694-744), (pTyr723) antibody (ABIN683788) Staining of canine lymph node tissue with ABIN683788. The primary antibody ABIN683788...