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anti-Human EPH Receptor A2 antibody for Immunofluorescence

Recommended EPH Receptor A2 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor A2 (EPHA2) Antibodies
  • ARCC2
  • AW545284
  • CTPA
  • CTPP1
  • CTRCT6
  • Eck
  • ECK
  • epha2
  • EPHA2
  • MGC89505
  • Myk2
  • Sek-2
  • Sek2
Reactivity
Human
199
102
45
10
6
4
3
2
2
2
2
1
Host
Mouse
165
55
6
Clonality
Monoclonal
Conjugate
This EPH Receptor A2 antibody is un-conjugated
11
8
8
8
8
6
2
2
2
2
2
2
2
2
2
Application
Cell-ELISA (cELISA), Flow Cytometry (FACS), Immunofluorescence (IF)
168
109
40
32
26
23
18
16
6
6
4
4
2
1
Supplier
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Catalog No. ABIN179724
$ 358.05
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3062192 ABIN2164634 IF ELISA WB Rabbit IgG Tyr588, Tyr596 Log in to see Polyclonal
0.3062192 ABIN2164633 IF ELISA WB Rabbit IgG Tyr588, Tyr596 Log in to see Polyclonal
0.3062192 ABIN2164637 IF IHC ELISA WB Rabbit IgG Tyr594, Tyr604 Log in to see Polyclonal
0.3062192 ABIN515205 IF ELISA WB Mouse IgG3 kappa AA 204-326, partial Log in to see 1E3
0.0010883404 ABIN488132 EIA FACS IF Mouse IgG1 Log in to see GM5H5 4
0.0010883404 ABIN498123 IF WB Rabbit Log in to see Polyclonal
0.0010883404 ABIN393385 IF WB Mouse IgG3 kappa AA 204-326 Log in to see 1E3
0.0010883404 ABIN2891870 IF WB Rabbit Thr587, Thr595 Log in to see Polyclonal
0.0010883404 ABIN3198051 IC IF WB Rabbit pTyr588, pTyr596 Log in to see Polyclonal
0.0010883404 ABIN3198052 IC IF WB Rabbit Log in to see Polyclonal
0.0010883404 ABIN1978825 IF IHC (p) WB Rabbit IgG AA 150-200 Log in to see Polyclonal
0.0010883404 ABIN1978826 IF IHC (p) Rabbit IgG AA 150-200 Log in to see Polyclonal
0.0010883404 ABIN2260620 IF ELISA WB Mouse IgG3 kappa AA 204-326 Log in to see 1E3
0.0010883404 ABIN2972585 ICC IF WB Rabbit Center, pTyr588, pTyr596 Log in to see Polyclonal
0.0010883404 ABIN2972587 ICC IF WB Rabbit Center Log in to see Polyclonal
0.0010883404 ABIN2257254 FACS IF ELISA Mouse IgG1 Log in to see 9E89

Similar anti-EPH Receptor A2 Antibodies

Application / Reactivity Human
Cell-ELISA (cELISA) 1 Antibodies
Dot Blot (DB) 18 Antibodies
ELISA 92 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 23 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 41 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 151 Antibodies

General

Antigen EPH Receptor A2 (EPHA2) Antibodies
Reactivity Human
(199), (102), (45), (10), (6), (4), (3), (2), (2), (2), (2), (1)
Host Mouse
(165), (55), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EPH Receptor A2 antibody is un-conjugated
(11), (8), (8), (8), (8), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Cell-ELISA (cELISA), Flow Cytometry (FACS), Immunofluorescence (IF)
(168), (109), (40), (32), (26), (23), (18), (16), (6), (6), (4), (4), (2), (1)
Pubmed 4 references available
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Product Details anti-EPH Receptor A2 Antibody

Target Details EPH Receptor A2 Application Details Handling References for anti-EPH Receptor A2 Antibody (ABIN179724) Images
Specificity Anti-human EphA2
Purification Protein G
Immunogen genetic immunisation with cDNA encoding human EphA2
Clone Kalpha-5H5
Isotype IgG1

Target Details EPH Receptor A2

Product Details anti-EPH Receptor A2 Antibody Application Details Handling References for anti-EPH Receptor A2 Antibody (ABIN179724) Images back to top
Antigen
Alternative Name EPHA2 (EPHA2 Antibody Abstract)
Background EphA2 (Ephrin receptor tyrosine kinase A2) belongs to the Ephrin tyrosine receptor familiy, the largest receptor tyrosine kinase family of transmembrane proteins. It encodes a 130 kDA transmembrane protein which is primarily found in adult human epithelial cells. Eph receptors and their ephrin ligands are important mediators of cell-cell communication and play roles in embryonic patterning, neuronal targeting, and vascular development during normal embryogenesis. The Eph family of receptor tyrosine kinases is frequently overexpressed in a wide variety of cancers and tumor cell lines. In particular, EphA2 is overexpressed in prostate, lung and colon cancers and 40% of breast cancers and it represent an attractive potential target for drug design.
UniProt P29317
Pathways RTK Signaling

Application Details

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Handling References for anti-EPH Receptor A2 Antibody (ABIN179724) Images back to top
Application Notes Flow cytometry: 1.2 µg/10^6 cells
Immunofluorescence: 1 µg/10^6 cells
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.
Comment

Synonyms: Eph receptor tyrosine kinase A2

Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details References for anti-EPH Receptor A2 Antibody (ABIN179724) Images back to top
Concentration 2 mg/mL
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C

References for anti-EPH Receptor A2 Antibody (ABIN179724)

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details Handling Images back to top
Background publications

Ireton, Chen: "EphA2 receptor tyrosine kinase as a promising target for cancer therapeutics." in: Current cancer drug targets, Vol. 5, Issue 3, pp. 149-57, 2005

Brantley-Sieders, Schmidt, Parker, Chen: "Eph receptor tyrosine kinases in tumor and tumor microenvironment." in: Current pharmaceutical design, Vol. 10, Issue 27, pp. 3431-42, 2004

Nakamoto, Bergemann: "Diverse roles for the Eph family of receptor tyrosine kinases in carcinogenesis." in: Microscopy research and technique, Vol. 59, Issue 1, pp. 58-67, 2002

Lindberg, Hunter: "cDNA cloning and characterization of eck, an epithelial cell receptor protein-tyrosine kinase in the eph/elk family of protein kinases." in: Molecular and cellular biology, Vol. 10, Issue 12, pp. 6316-24, 1991

Images

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details Handling References for anti-EPH Receptor A2 Antibody (ABIN179724) back to top
Supplier Images
 image for anti-EPH Receptor A2 antibody (EPHA2) (ABIN179724) FACS analysis of BOSC23 cells using Kalpha-5H5. BOSC23 cells were transiently trans-f...
 image for anti-EPH Receptor A2 antibody (EPHA2) (ABIN179724) Spectral Confocal Microscopy of CHO cells using K?-5H5. CHO cells were transiently tr...
 image for anti-EPH Receptor A2 antibody (EPHA2) (ABIN179724) SDS-PAGE analysis of purified K?-5H5 monoclonal antibody. Lane 1: molecular weight ma...
SDS-PAGE (SDS) image for anti-EPH Receptor A2 antibody (EPHA2) (ABIN179724) SDS-PAGE analysis of purified Kα-5H5 monoclonal antibody. Lane 1: molecular weight ma...
Flow Cytometry (FACS) image for anti-EPH Receptor A2 antibody (EPHA2) (ABIN179724) FACS analysis of BOSC23 cells using Kα-5H5. BOSC23 cells were transiently trans-fecte...
Immunofluorescence (IF) image for anti-EPH Receptor A2 antibody (EPHA2) (ABIN179724) Spectral Confocal Microscopy of CHO cells using Kα-5H5. CHO cells were transiently tr...