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|Application / Reactivity||Rat (Rattus)||Human|
|ELISA||5 Antibodies||131 Antibodies|
|Immunofluorescence (IF)||4 Antibodies||17 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||23 Antibodies||23 Antibodies|
|Immunohistochemistry (IHC)||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||11 Antibodies|
|Western Blotting (WB)||18 Antibodies|
|Cell-ELISA (cELISA)||1 Antibodies|
|Dot Blot (DB)||18 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||48 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunochromatography (IC)||4 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Antigen||EPH Receptor A2 (EPHA2) Antibodies|
|Epitope||AA 954-976, C-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This EPH Receptor A2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-EPH Receptor A2 AntibodyTarget Details EPH Receptor A2 Application Details Handling Images
|Sequence||HQKRIAYSLL GLKDQVNTVG IPI|
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Ephrin type-A receptor 2(EPHA2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: EPH receptor A2
Protein Name: Ephrin type-A receptor 2
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Eph receptor A2(954-976aa HQKRIAYSLLGLKDQVNTVGIPI), identical to the related rat and mouse sequences.|
Target Details EPH Receptor A2Product Details anti-EPH Receptor A2 Antibody Application Details Handling Images back to top
|Alternative Name||EPHA2 (EPHA2 Antibody Abstract)|
|Background||EPHA2(ephrin type-A receptor 2) also known as ECK, is a protein that in humans is encoded by the EPHA2 gene. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. By somatic cell hybrid analysis and fluorescence in situ hybridization, the EPHA2 gene is mapped to chromosome 1p36.1. By screening a HeLa cell cDNA library with degenerate oligonucleotides based on highly conserved regions of receptor protein-tyrosine kinases, Lindberg and Hunter isolated cDNAs encoding EPHA2, which they called ECK. EPHA2 was readily detectable in human lens fiber cells using immunoblot and immunohistochemistry. EGFR and EPHA2 mediated HCV entry by regulating CD81 -claudin-1(CLDN1) coreceptor associations and viral glycoprotein-dependent membrane fusion.|
Application DetailsProduct Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
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