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anti-Rat (Rattus) EPH Receptor A2 antibody for Western Blotting

Recommended EPH Receptor A2 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor A2 (EPHA2) Antibodies
  • ARCC2
  • AW545284
  • CTPA
  • CTPP1
  • CTRCT6
  • Eck
  • ECK
  • epha2
  • EPHA2
  • MGC89505
  • Myk2
  • Sek-2
  • Sek2
Epitope
AA 954-976, C-Term
27
20
18
18
18
17
15
13
13
11
9
7
7
6
6
6
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
247
111
44
10
6
5
4
4
4
2
2
2
1
Host
Rabbit
188
80
14
Clonality
Polyclonal
Conjugate
This EPH Receptor A2 antibody is un-conjugated
13
10
10
10
8
6
6
5
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
210
148
59
53
42
23
18
17
6
6
4
4
2
1
1
1
Supplier
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Catalog No. ABIN3044136
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN498123 IF WB Rabbit Log in to see Polyclonal
0.13341247 ABIN2706107 WB Rabbit pTyr588 Log in to see Polyclonal
0.13341247 ABIN2164637 IF IHC ELISA WB Rabbit IgG Tyr594, Tyr604 Log in to see Polyclonal
0.0011728023 ABIN669201 IF (p) IHC (p) WB Rabbit IgG AA 920-970 Log in to see Polyclonal 1
0.0011728023 ABIN372265 IHC (p) WB Rabbit IgG AA 450-500 Log in to see Polyclonal
0.0011728023 ABIN684508 IF (p) IHC (p) WB Rabbit IgG AA 570-610, pTyr594 Log in to see Polyclonal
0.0011728023 ABIN1584404 ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0011728023 ABIN669203 IHC (p) WB Biotin Rabbit IgG AA 920-970 Log in to see Polyclonal
0.0011728023 ABIN669210 IHC (p) WB HRP Rabbit IgG AA 920-970 Log in to see Polyclonal
0.0011728023 ABIN2469826 IHC WB Rabbit AA 450-500 Log in to see Polyclonal
0.0011728023 ABIN1734074 IHC (p) WB Rabbit IgG AA 450-500 Log in to see Polyclonal
0.0011728023 ABIN3198051 IC IF WB Rabbit pTyr588, pTyr596 Log in to see Polyclonal
0.0011728023 ABIN3198052 IC IF WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN2562444 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN2416393 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN3030816 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.0011728023 ABIN3048645 IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-EPH Receptor A2 antibody for Western Blotting

Similar anti-EPH Receptor A2 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 5 Antibodies 131 Antibodies
Immunofluorescence (IF) 4 Antibodies 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Western Blotting (WB) 18 Antibodies
Cell-ELISA (cELISA) 1 Antibodies
Dot Blot (DB) 18 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 48 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 4 Antibodies

General

Antigen EPH Receptor A2 (EPHA2) Antibodies
Epitope AA 954-976, C-Term
(27), (20), (18), (18), (18), (17), (15), (13), (13), (11), (9), (7), (7), (6), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(247), (111), (44), (10), (6), (5), (4), (4), (4), (2), (2), (2), (1)
Host Rabbit
(188), (80), (14)
Clonality
Polyclonal
Conjugate This EPH Receptor A2 antibody is un-conjugated
(13), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(210), (148), (59), (53), (42), (23), (18), (17), (6), (6), (4), (4), (2), (1), (1), (1)
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Product Details anti-EPH Receptor A2 Antibody

Target Details EPH Receptor A2 Application Details Handling Images
Sequence HQKRIAYSLL GLKDQVNTVG IPI
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Ephrin type-A receptor 2(EPHA2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: EPH receptor A2
Protein Name: Ephrin type-A receptor 2
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Eph receptor A2(954-976aa HQKRIAYSLLGLKDQVNTVGIPI), identical to the related rat and mouse sequences.
Isotype IgG

Target Details EPH Receptor A2

Product Details anti-EPH Receptor A2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name EPHA2 (EPHA2 Antibody Abstract)
Background EPHA2(ephrin type-A receptor 2) also known as ECK, is a protein that in humans is encoded by the EPHA2 gene. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. By somatic cell hybrid analysis and fluorescence in situ hybridization, the EPHA2 gene is mapped to chromosome 1p36.1. By screening a HeLa cell cDNA library with degenerate oligonucleotides based on highly conserved regions of receptor protein-tyrosine kinases, Lindberg and Hunter isolated cDNAs encoding EPHA2, which they called ECK. EPHA2 was readily detectable in human lens fiber cells using immunoblot and immunohistochemistry. EGFR and EPHA2 mediated HCV entry by regulating CD81 -claudin-1(CLDN1) coreceptor associations and viral glycoprotein-dependent membrane fusion.
UniProt P29317
Pathways RTK Signaling

Application Details

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-EPH Receptor A2 antibody (EPHA2) (AA 954-976) (ABIN3044136) Anti-Eph receptor A2 antibody, IHC(P) IHC(P): Human Lung Cancer Tissue
Western Blotting (WB) image for anti-EPH Receptor A2 antibody (EPHA2) (AA 954-976) (ABIN3044136) Anti-Eph receptor A2 antibody, Western blotting Lane 1: Rat Ovary Tissue Lysate Lane ...