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anti-Human EPH Receptor B2 antibody for Western Blotting

Recommended EPH Receptor B2 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor B2 (EPHB2) Antibodies
  • CAPB
  • Cek5
  • DRT
  • Drt
  • EK5
  • EPHT3
  • Erk
  • ERK
  • ETECK
  • Hek5
  • Nuk
  • PCBC
  • Prkm5
  • Qek5
  • RGD1564232
  • Sek3
  • Tyro5
Epitope
AA 1021-1050, C-Term
30
20
15
14
10
8
7
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
131
29
4
3
3
3
2
2
1
1
1
1
1
1
Host
Rabbit
77
38
18
8
Clonality
Polyclonal
Conjugate
This EPH Receptor B2 antibody is un-conjugated
9
6
6
6
6
6
2
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
108
88
51
25
22
21
11
7
1
1
1
1
Supplier
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Catalog No. ABIN391920
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12471054 ABIN359813 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 5
0.12471054 ABIN359812 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 5
0.12471054 ABIN359814 EIA IHC (p) WB Rabbit Ig Log in to see Polyclonal 5
0.12471054 ABIN1533687 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12471054 ABIN391921 FACS IHC (p) WB Rabbit Ig Log in to see Polyclonal 3
0.12471054 ABIN387812 FACS IHC (p) WB Mouse IgG1 kappa Log in to see 48CT12-6-4 5
0.12471054 ABIN1724695 ICC ELISA WB Mouse IgG2b AA 17-200 Log in to see 2D12C6 2
0.12471054 ABIN356401 EIA IHC (p) WB Mouse IgG1 Log in to see 48CT12-6-4
0.12471054 ABIN614403 EIA IF IHC (p) WB Mouse IgG2b AA 17-200 Log in to see 2D12C6
0.12471054 ABIN188674 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
0.12471054 ABIN2465658 ELISA WB Goat Internal Region Log in to see Polyclonal
0.12471054 ABIN2164659 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.12471054 ABIN2164662 ELISA WB Rabbit IgG Tyr596, Tyr600, Tyr602, Tyr614 Log in to see Polyclonal
0.12471054 ABIN2160961 IF IHC WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.12471054 ABIN515343 ELISA WB Mouse IgG2a kappa AA 226-325, partial Log in to see 4D1
0.12471054 ABIN3184511 ELISA IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.12471054 ABIN1107110 EIA IF WB Mouse IgG2b Log in to see 2D12C6
0.12471054 ABIN2158722 FACS IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.12471054 ABIN2473465 WB Mouse IgG2a AA 226-325 Log in to see 4D1
0.12471054 ABIN1843505 ICC IF ELISA WB Mouse IgG2b AA 17-200 Log in to see

Top referenced anti-EPH Receptor B2 antibody for Western Blotting

Similar anti-EPH Receptor B2 Antibodies

Application / Reactivity Human
ELISA 85 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 18 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 22 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 51 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 107 Antibodies

General

Antigen EPH Receptor B2 (EPHB2) Antibodies
Epitope AA 1021-1050, C-Term
(30), (20), (15), (14), (10), (8), (7), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(131), (29), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(77), (38), (18), (8)
Clonality (Clone)
Polyclonal   ( )
Conjugate This EPH Receptor B2 antibody is un-conjugated
(9), (6), (6), (6), (6), (6), (2)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(108), (88), (51), (25), (22), (21), (11), (7), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-EPH Receptor B2 Antibody

Target Details EPH Receptor B2 Application Details Handling References for anti-EPH Receptor B2 Antibody (ABIN391920) Images
Specificity This EphB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1026~1055 amino acids from the C-terminal region of human EphB2.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This EphB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1021-1050 AA from the C-terminal region of human EphB2.
Clone RB01652
Isotype Ig

Target Details EPH Receptor B2

Product Details anti-EPH Receptor B2 Antibody Application Details Handling References for anti-EPH Receptor B2 Antibody (ABIN391920) Images back to top
Antigen
Alternative Name EphB2 (EPHB2 Antibody Abstract)
Background Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. It binds RASGAP through the juxtamembrane tyrosines residues, and also interacts with PRKCABP and GRIP1 This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. This protein contains putatively 2 fibronectin type III domains and 1 sterile alpha motif (SAM) domain.
Synonyms: Ephrin type-B receptor 2, Tyrosine-protein kinase receptor EPH-3, DRT, Receptor protein-tyrosine kinase HEK5, ERK
Molecular Weight 117506 DA
Gene ID 2048
UniProt P29323
Research Area Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure, Neurology
Pathways RTK Signaling

Application Details

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Handling References for anti-EPH Receptor B2 Antibody (ABIN391920) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50, IF = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details References for anti-EPH Receptor B2 Antibody (ABIN391920) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-EPH Receptor B2 Antibody (ABIN391920)

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details Handling Images back to top
Background publications

Thanos, Goodwill, Bowie: "Oligomeric structure of the human EphB2 receptor SAM domain." in: Science (New York, N.Y.), Vol. 283, Issue 5403, pp. 833-6, 1999

Ikegaki, Tang, Liu, Biegel, Allen, Yoshioka, Sulman, Brodeur, Pleasure: "Molecular characterization and chromosomal localization of DRT (EPHT3): a developmentally regulated human protein-tyrosine kinase gene of the EPH family." in: Human molecular genetics, Vol. 4, Issue 11, pp. 2033-45, 1996

Iwase, Tanaka, Suzuki, Naito, Sugimura, Kino: "Identification of protein-tyrosine kinase genes preferentially expressed in embryo stomach and gastric cancer." in: Biochemical and biophysical research communications, Vol. 194, Issue 2, pp. 698-705, 1993

Images

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details Handling References for anti-EPH Receptor B2 Antibody (ABIN391920) back to top
Supplier Images
Western Blotting (WB) image for anti-EPH Receptor B2 antibody (EPHB2) (AA 1021-1050) (ABIN391920) Western blot analysis of anti-EphB2 C-term Pab (ABIN391920) in NCI-H460 cell lysate. ...
Immunohistochemistry (IHC) image for anti-EPH Receptor B2 antibody (EPHB2) (AA 1021-1050) (ABIN391920) Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EphB2 Antibo...
Immunohistochemistry (IHC) image for anti-EPH Receptor B2 antibody (EPHB2) (AA 1021-1050) (ABIN391920) Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EphB2...