You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) EPH Receptor B2 antibody for Immunohistochemistry (Frozen Sections)

Recommended EPH Receptor B2 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor B2 (EPHB2) Antibodies
  • CAPB
  • Cek5
  • DRT
  • Drt
  • EK5
  • EPHT3
  • Erk
  • ERK
  • ETECK
  • Hek5
  • Nuk
  • PCBC
  • Prkm5
  • Qek5
  • RGD1564232
  • Sek3
  • Tyro5
Reactivity
Human, Mouse (Murine)
142
34
4
3
3
3
2
2
1
1
1
1
1
1
Host
Mouse
81
38
19
14
Clonality
Monoclonal
Conjugate
This EPH Receptor B2 antibody is un-conjugated
9
6
6
6
6
6
2
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
114
93
52
30
23
23
12
7
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN2689499
Contact our Customer Service for availability and price in your country.

Top referenced anti-EPH Receptor B2 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-EPH Receptor B2 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 9 Antibodies 89 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 7 Antibodies
Flow Cytometry (FACS) 14 Antibodies 24 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 10 Antibodies
Immunofluorescence (IF) 1 Antibodies 23 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Western Blotting (WB) 15 Antibodies

General

Antigen EPH Receptor B2 (EPHB2) Antibodies
Reactivity Human, Mouse (Murine)
(142), (34), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(81), (38), (19), (14)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EPH Receptor B2 antibody is un-conjugated
(9), (6), (6), (6), (6), (6), (2), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(114), (93), (52), (30), (23), (23), (12), (7), (1), (1), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-EPH Receptor B2 Antibody

Target Details EPH Receptor B2 Application Details Handling References for anti-EPH Receptor B2 Antibody (ABIN2689499) Images
Brand BD Pharmingen™
Characteristics The 2H9 monoclonal antibody specifically binds to the Ephrin Type-B Receptor 2 (EphB2). EphB2 is a type I transmembrane glycoprotein that belongs to the Eph receptor family of tyrosine kinase receptors. EphB2 serves as a cell surface receptor tyrosine kinase for membrane-anchored ligands referred to as type B ephrins (ephrin-B). The EphB2 receptor can bind to ephrin-B1, ephrin-B2, and ephrin-B3. Transmembrane ephrin-B family members are key regulators of embryogenesis including development of the nervous and vascular systems. The EphB2 receptor functions as a chemodirectant in regulating cellular migration. EphB2/ephrin-B interactions orchestrate cell positioning by regulating cellular adhesion and repulsion during development, thereby influencing cell fate, morphogenesis and organogenesis. Signaling can occur in a forward pathway when the EphB2 receptor tyrosine kinase is activated by bound ligand and in a reverse pathway when transmembrane ephrin-B ligands are activated by EphB2 receptor-mediated crosslinking. In the adult body, Eph receptor signaling plays major roles in regulating the architecture and physiology of different tissues under normal as well as disease conditions such as cancer. Ephrin-B1 and ephrin-B2 levels are upregulated in the vasculature during inflammation. Ephrin-B2 Molecules that are localized to the luminal endothelial surface can signal through the EphB2 which is expressed by monocytes. This interaction promotes monocyte differentiation into proinflammatory macrophages. In the intestinal epithelium, EphB2/ephrin-B interactions regulate both cell positioning and tumor progression. The differential expression patterns of EphB2 allows for the detection and isolation of various intestinal epithelial cell types. These include intestinal stem cells (ISCs) which express high levels of EphB2. The 2H9 antibody reportedly blocks the interaction of EphB2 with ephrin ligands and crossreacts with mouse EphB2. Flow cytometric analysis of EphB2 expression on human Colo-205 cells. Cells from the human COLO 205 (Colorectal adenocarcinoma, ATCC CCL-222) cell line were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 550878, dashed line histogram) or Purified Mouse Anti-Human EphB2 antibody (Cat. No. 564698, solid line histogram). The cells were washed and counterstained with PE Goat Anti-Mouse Ig (Cat. No. 550589). The fluorescence histograms showing EphB2 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. Immunohistochemical analysis of human EphB2 expression in human placenta. Acetone-fixed frozen human placenta sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat No.550878, Left Image) or Purified Mouse Anti-Human EphB2 antibody (Right Image). A three-step staining procedure that employed Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification:20x. 564698 Rev. 1 Page 1 of 2

BD Pharmingen™ Purified Mouse Anti-Human EphB2 - Purified - Clone 2H9 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human EphB2 Recombinant Protein
Clone 2H9
Isotype IgG1, kappa

Target Details EPH Receptor B2

Product Details anti-EPH Receptor B2 Antibody Application Details Handling References for anti-EPH Receptor B2 Antibody (ABIN2689499) Images back to top
Antigen
Alternative Name EphB2 (EPHB2 Antibody Abstract)
Background Synonyms: EPHB2, EphB2R, TYRO5, CAPB, DRT, EK5, EPHT3, EPTH3, HEK5, PCBC
Pathways RTK Signaling

Application Details

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Handling References for anti-EPH Receptor B2 Antibody (ABIN2689499) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details References for anti-EPH Receptor B2 Antibody (ABIN2689499) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-EPH Receptor B2 Antibody (ABIN2689499)

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details Handling Images back to top
Product cited in:

Jung, Sato, Merlos-Suárez, Barriga, Iglesias, Rossell, Auer, Gallardo, Blasco, Sancho, Clevers, Batlle: "Isolation and in vitro expansion of human colonic stem cells." in: Nature medicine, Vol. 17, Issue 10, pp. 1225-7, 2011

Merlos-Suárez, Barriga, Jung, Iglesias, Céspedes, Rossell, Sevillano, Hernando-Momblona, da Silva-Diz, Muñoz, Clevers, Sancho, Mangues, Batlle: "The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse." in: Cell stem cell, Vol. 8, Issue 5, pp. 511-24, 2011

Foster, Gordon, Cardenas, Veiga-Fernandes, Makinen, Grigorieva, Wilkinson, Blackburn, Richie, Manley, Adams, Kioussis, Coles: "EphB-ephrin-B2 interactions are required for thymus migration during organogenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 30, pp. 13414-9, 2010

Mao, Luis, Ross, Silva, Tan, Crowley, Chui, Franz, Senter, Koeppen, Polakis: "EphB2 as a therapeutic antibody drug target for the treatment of colorectal cancer." in: Cancer research, Vol. 64, Issue 3, pp. 781-8, 2004