You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) EPH Receptor B2 antibody for Western Blotting

Recommended EPH Receptor B2 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor B2 (EPHB2) Antibodies
  • CAPB
  • DRT
  • EK5
  • EPHT3
  • ERK
  • Hek5
  • PCBC
  • Tyro5
  • Cek5
  • Drt
  • ETECK
  • Erk
  • Nuk
  • Prkm5
  • Qek5
  • Sek3
  • RGD1564232
  • EPH receptor B2
  • Eph receptor B2
  • EPHB2
  • LOC100218346
  • Ephb2
Epitope
N-Term
25
18
14
14
14
10
9
7
5
5
4
3
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
158
57
5
3
3
3
3
3
2
2
1
1
1
1
1
Host
Rabbit
79
41
25
20
Clonality
Polyclonal
Conjugate
This EPH Receptor B2 antibody is un-conjugated
10
6
6
6
6
6
3
2
2
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
115
90
51
43
27
25
15
6
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN359812
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0923712 ABIN4903589 WB Rabbit IgG Log in to see Polyclonal
3.0923712 ABIN2562445 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
3.0923712 ABIN2473465 WB Mouse IgG2a AA 226-325 Log in to see 4D1
1 ABIN4900040 FACS ICC IHC WB Goat IgG AA 27-548 Log in to see Polyclonal 19
1 ABIN730708 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN291908 ELISA WB Goat AA 882-893 Log in to see Polyclonal
1 ABIN958114 ICC FACS IHC WB Goat IgG AA 27-548 Log in to see Polyclonal
1 ABIN1583645 ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN3198053 WB Rabbit C-Term Log in to see Polyclonal
1 ABIN200836 IP ELISA WB Mouse IgG1, kappa C-Term Log in to see
1 ABIN1963152 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1965529 IHC ELISA WB APC Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1971938 IHC ELISA WB FITC Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1969602 IHC ELISA WB Biotin Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1976533 IHC ELISA WB PE Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN603141 ELISA WB Rat IgG1 AA 27-551 Log in to see
1 ABIN1974197 IHC ELISA WB HRP Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN2620454 ELISA IHC WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN603139 ELISA WB Mouse IgG AA 226-325 Log in to see
1 ABIN1831710 ICC FACS ELISA WB Goat IgG Log in to see Polyclonal

Top referenced anti-EPH Receptor B2 antibody for Western Blotting

Similar anti-EPH Receptor B2 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 17 Antibodies 87 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 7 Antibodies
Flow Cytometry (FACS) 27 Antibodies 39 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 10 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 26 Antibodies
Immunofluorescence (IF) 27 Antibodies

General

Antigen EPH Receptor B2 (EPHB2) Antibodies
Epitope N-Term
(25), (18), (14), (14), (14), (10), (9), (7), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(158), (57), (5), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(79), (41), (25), (20)
Clonality
Polyclonal
Conjugate This EPH Receptor B2 antibody is un-conjugated
(10), (6), (6), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(115), (90), (51), (43), (27), (25), (15), (6), (2), (1), (1), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-EPH Receptor B2 Antibody

Target Details EPH Receptor B2 Application Details Handling References for anti-EPH Receptor B2 Antibody (ABIN359812) Images
Specificity This antibody reacts to EphB2.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between aa 111 - 140 from the N-terminal region of human EphB2.
Isotype Ig

Target Details EPH Receptor B2

Product Details anti-EPH Receptor B2 Antibody Application Details Handling References for anti-EPH Receptor B2 Antibody (ABIN359812) Images back to top
Antigen
Alternative Name EPHB2 (EPHB2 Antibody Abstract)
Background Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. It binds RASGAP through the juxtamembrane tyrosines residues, and also interacts with PRKCABP and GRIP1 This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. This protein contains putatively 2 fibronectin type III domains and 1 sterile alpha motif (SAM) domain.Synonyms: DRT, DRT, EPHT3, EPTH3, ERK, Ephrin type-B receptor 2, HEK5, NY-REN-47, Receptor protein-tyrosine kinase HEK5, Renal carcinoma antigen NY-REN-47, TYRO5, Tyrosine-protein kinase TYRO5, Tyrosine-protein kinase receptor EPH-3
Gene ID 2048, 9606
UniProt P29323
Pathways RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Handling References for anti-EPH Receptor B2 Antibody (ABIN359812) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details References for anti-EPH Receptor B2 Antibody (ABIN359812) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-EPH Receptor B2 Antibody (ABIN359812)

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details Handling Images back to top
Background publications

Thanos, Goodwill, Bowie: "Oligomeric structure of the human EphB2 receptor SAM domain." in: Science (New York, N.Y.), Vol. 283, Issue 5403, pp. 833-6, 1999

Tang, Pleasure, Brodeur, Ikegaki: "A variant transcript encoding an isoform of the human protein tyrosine kinase EPHB2 is generated by alternative splicing and alternative use of polyadenylation signals." in: Oncogene, Vol. 17, Issue 4, pp. 521-6, 1998

Ikegaki, Tang, Liu, Biegel, Allen, Yoshioka, Sulman, Brodeur, Pleasure: "Molecular characterization and chromosomal localization of DRT (EPHT3): a developmentally regulated human protein-tyrosine kinase gene of the EPH family." in: Human molecular genetics, Vol. 4, Issue 11, pp. 2033-45, 1996

Fox, Holst, Chute, Lindberg, Janssen, Basu, Welcher: "cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases." in: Oncogene, Vol. 10, Issue 5, pp. 897-905, 1995

Iwase, Tanaka, Suzuki, Naito, Sugimura, Kino: "Identification of protein-tyrosine kinase genes preferentially expressed in embryo stomach and gastric cancer." in: Biochemical and biophysical research communications, Vol. 194, Issue 2, pp. 698-705, 1993

Images

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details Handling References for anti-EPH Receptor B2 Antibody (ABIN359812) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EPH Receptor B2 antibody (EPHB2) (N-Term) (ABIN359812) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-EPH Receptor B2 antibody (EPHB2) (N-Term) (ABIN359812) Western blot analysis of anti-EphB2 Pab in mouse heart tissue. EphB2 (Arrow) was dete...