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anti-Human EPH Receptor B4 antibody for Flow Cytometry

Recommended EPH Receptor B4 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor B4 (EPHB4) Antibodies
  • htk
  • myk1
  • ephB4a
  • tyro11
  • ephb4b
  • MGC130997
  • AI042935
  • Htk
  • MDK2
  • Myk1
  • Tyro11
  • HTK
  • MYK1
  • TYRO11
  • EPH receptor B4
  • Eph receptor B4
  • EPHB4
  • ephb4-a
  • ephb4
  • LOC100359286
  • Ephb4
Epitope
AA 16-539
20
18
16
14
12
7
6
5
4
3
3
3
3
2
1
1
1
1
Reactivity
Human
142
54
10
1
1
1
1
Host
Goat
67
65
25
17
Clonality
Polyclonal
Conjugate
This EPH Receptor B4 antibody is un-conjugated
7
6
6
4
4
4
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
117
101
34
25
20
15
5
4
4
3
2
1
1
Supplier
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Catalog No. ABIN4899281
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.207517 ABIN1996162 FACS IF IF/ICC FITC Mouse IgG1 AA 1-539 Log in to see 04
14.207517 ABIN1996164 FACS IF IF/ICC PE Mouse IgG1 AA 1-539 Log in to see 04
14.207517 ABIN1996166 FACS FITC Rabbit IgG AA 1-539 Log in to see 001
14.207517 ABIN1996168 FACS PE Rabbit IgG AA 1-539 Log in to see 001
14.207517 ABIN2213793 FACS APC Mouse IgG1 AA 1-539 Log in to see 04
14.207517 ABIN4895853 FACS Fluorescein Rat IgG1 AA 16-539 Log in to see 395810
14.207517 ABIN4895854 FACS Alexa Fluor 700 Rat IgG1 AA 16-539 Log in to see 395810
14.207517 ABIN4895855 FACS PE Rat IgG1 AA 16-539 Log in to see 395810
14.207517 ABIN4895851 FACS Allophycocyanin Rat IgG1 AA 16-539 Log in to see 395810
14.207517 ABIN1996159 ELISA FACS IF IF/ICC Mouse IgG1 AA 1-539, Extracellular Domain Log in to see 04
14.207517 ABIN2213795 FACS APC Rabbit IgG AA 1-539 Log in to see 001
14.207517 ABIN4895852 FACS PerCP Rat IgG1 AA 16-539 Log in to see 395810
14.207517 ABIN4899280 FACS WB Rat IgG1 AA 16-539 Log in to see 395810
14.207517 ABIN2681935 ELISA FACS Rabbit IgG AA 1-539 Log in to see 001
1 ABIN958115 FACS IHC (p) WB Goat IgG AA 16-539 Log in to see Polyclonal
1 ABIN1872556 FACS IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN4308724 FACS Alexa Fluor 350 Rat IgG1 AA 16-539 Log in to see 395810
1 ABIN4308725 FACS Alexa Fluor 405 Rat IgG1 AA 16-539 Log in to see 395810
1 ABIN4308726 FACS Alexa Fluor 750 Rat IgG1 AA 16-539 Log in to see 395810
1 ABIN4308721 FACS Alexa Fluor 488 Rat IgG1 AA 16-539 Log in to see 395810

Top referenced anti-EPH Receptor B4 antibody for Flow Cytometry

Similar anti-EPH Receptor B4 Antibodies

Application / Reactivity Human
ELISA 83 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 30 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 16 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunohistochemistry (IHC) 21 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 91 Antibodies

General

Antigen EPH Receptor B4 (EPHB4) Antibodies
Epitope AA 16-539
(20), (18), (16), (14), (12), (7), (6), (5), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Reactivity Human
(142), (54), (10), (1), (1), (1), (1)
Host Goat
(67), (65), (25), (17)
Clonality
Polyclonal
Conjugate This EPH Receptor B4 antibody is un-conjugated
(7), (6), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
(117), (101), (34), (25), (20), (15), (5), (4), (4), (3), (2), (1), (1)
Pubmed 6 references available
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Product Details anti-EPH Receptor B4 Antibody

Target Details EPH Receptor B4 Application Details Handling References for anti-EPH Receptor B4 Antibody (ABIN4899281) Images
Specificity Detects human EphB4 in direct ELISAs and Western blots. In direct ELISAs, less than 10 % cross-reactivity with recombinant mouse (rm) EphB4 is observed and less than 1 % cross-reactivity with recombinant human (rh) EphA4, rhEphA3, rhEphB2, and rhEphB3 is observed.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human EphB4, Leu16-Ala539, Accession # P54760
Isotype IgG

Target Details EPH Receptor B4

Product Details anti-EPH Receptor B4 Antibody Application Details Handling References for anti-EPH Receptor B4 Antibody (ABIN4899281) Images back to top
Antigen
Alternative Name EphB4 (EPHB4 Antibody Abstract)
Background Alternative Names: EC 2.7.10, EC 2.7.10.1, EPH receptor B4, EphB4, ephrin type-B receptor 4, hepatoma transmembrane kinase, Htk, HTKephrin receptor EphB4, Mdk2, Myk1, soluble EPHB4 variant 1, soluble EPHB4 variant 2, soluble EPHB4 variant 3, Tyro11, Tyrosine-protein kinase receptor HTK, Tyrosine-protein kinase TYRO11
Gene ID 2050
UniProt P54760
Pathways RTK Signaling

Application Details

Product Details anti-EPH Receptor B4 Antibody Target Details EPH Receptor B4 Handling References for anti-EPH Receptor B4 Antibody (ABIN4899281) Images back to top
Application Notes Western Blot 2 μg/mL
Simple Western 50 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunohistochemistry 5-15 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor B4 Antibody Target Details EPH Receptor B4 Application Details References for anti-EPH Receptor B4 Antibody (ABIN4899281) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-EPH Receptor B4 Antibody (ABIN4899281)

Product Details anti-EPH Receptor B4 Antibody Target Details EPH Receptor B4 Application Details Handling Images back to top
Product cited in:

Fuggetta, Bordignon, Cottarelli, Macchi, Frezza, Cordiali-Fei, Ensoli, Ciafrè, Marino-Merlo, Mastino, Ravagnan: "Downregulation of proinflammatory cytokines in HTLV-1-infected T cells by Resveratrol." in: Journal of experimental & clinical cancer research : CR, Vol. 35, Issue 1, pp. 118, 2016 Method employed by authors: Immunoprecipitation (IP) (Sample species: Human).

Martin-Almedina, Martinez-Corral, Holdhus, Vicente, Fotiou, Lin, Petersen, Simpson, Hoischen, Gilissen, Jeffery, Atton, Karapouliou, Brice, Gordon, Wiseman, Wedin, Rockson, Jeffery, Mortimer, Snyder et al.: "EPHB4 kinase-inactivating mutations cause autosomal dominant lymphatic-related hydrops fetalis. ..." in: The Journal of clinical investigation, Vol. 126, Issue 8, pp. 3080-8, 2016 Method employed by authors: Immunoprecipitation (IP) (Sample species: Human).

Boyer-Di Ponio, El-Ayoubi, Glacial, Ganeshamoorthy, Driancourt, Godet, Perrière, Guillevic, Couraud, Uzan: "Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes." in: PLoS ONE, Vol. 9, Issue 1, pp. e84179, 2014 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Human).

He, Kumar, Zhou, Krasnoperov, Ryan, Gill, Hinton: "Soluble EphB4 inhibition of PDGF-induced RPE migration in vitro." in: Investigative ophthalmology & visual science, Vol. 51, Issue 1, pp. 543-52, 2010 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Human).

Suzuki, Abe, Imagama, Nomura, Tanizaki, Minami, Hayakawa, Ito, Katsumi, Yamamoto, Emi, Kiyoi, Naoe: "BCR-ABL-independent and RAS / MAPK pathway-dependent form of imatinib resistance in Ph-positive acute lymphoblastic leukemia cell line with activation of EphB4." in: European journal of haematology, Vol. 84, Issue 3, pp. 229-38, 2010 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Kwan Tat, Pelletier, Amiable, Boileau, Lajeunesse, Duval, Martel-Pelletier: "Activation of the receptor EphB4 by its specific ligand ephrin B2 in human osteoarthritic subchondral bone osteoblasts." in: Arthritis and rheumatism, Vol. 58, Issue 12, pp. 3820-30, 2008 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Images

Product Details anti-EPH Receptor B4 Antibody Target Details EPH Receptor B4 Application Details Handling References for anti-EPH Receptor B4 Antibody (ABIN4899281) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-EPH Receptor B4 antibody (EPHB4) (AA 16-539) (ABIN4899281) Detection of EphB4 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast can...
Immunohistochemistry (IHC) image for anti-EPH Receptor B4 antibody (EPHB4) (AA 16-539) (ABIN4899281) EphB4 in Human Kidney. EphB4 was detected in immersion fixed paraffin-embedded sectio...
Simple Western (SimWes) image for anti-EPH Receptor B4 antibody (EPHB4) (AA 16-539) (ABIN4899281) Detection of Human EphB4 by Simple WesternTM. Simple Western lane view shows lysates ...
Western Blotting (WB) image for anti-EPH Receptor B4 antibody (EPHB4) (AA 16-539) (ABIN4899281) Detection of Human EphB4 by Western Blot. Western blot shows lysates of K562 human ch...