EPH Receptor B4 antibody (Extracellular, N-Term)

Catalog No. ABIN7886302

The Rabbit Polyclonal anti-EPH Receptor B4 antibody (ABIN7886302) specifically detects EPH Receptor B4 in WB, IHC, IF, FACS and LCI. The antibody is reactive with Mouse samples.

Quick Overview for EPH Receptor B4 antibody (Extracellular, N-Term) (ABIN7886302)

Target: EPH Receptor B4 (EPHB4)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor B4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-EPH Receptor B4 Antibody

Binding Specificity: AA 472-486, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to EphB4 Receptor

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-EphB4 (extracellular) Antibody (ABIN7043127, ABIN7044253 and ABIN7044254) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize EphB4 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)HEKGAEGPSSVRFLK, corresponding to amino acid residues 472-486 of mouse EphB4

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5-5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235298)

Blocking Peptide: (ABIN7235298)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for EPH Receptor B4

Target: EPH Receptor B4 (EPHB4)

Alternative Name: EPHB4

Background:

Synonyms: Ephrin type-B receptor 4, Eph receptor B4, Hepatoma transmembrane kinase, HTK

Description: EphB4 (Ephrin type-B receptor 4) is a member of the Eph family of receptor tyrosine kinases. Signaling pathways of these receptors play important roles in regulating numerous physiological processes during development including cell morphology, proliferation, migration and axonal guidance, dendritic spine formation and neural plasticity. Ephrins transduce bidirectional signals based on their distribution on the communicating cell surfaces. Eph receptors mediate forward signaling in one cell while ephrin ligands transmit reverse signaling in an adjacent cell1,2.Eph receptors consist of two large subfamilies: EphAs and EphBs that bind to either glycophosphatidylinositol (GPI)-anchored ephrin-A ligands or transmembrane ephrin-B ligands respectively. Each eph receptor may interact with multiple ligands, except for EPHB4, which only binds ephrin-B22.EPHB4 is expressed primarily during embryonic development in neural tissue and immature vasculature. Studies show that EphB4 is implicated in the growth of several epithelial tumors and is a promising target in cancer therapy. EphB4 signaling was found to promote tumor growth, invasiveness, chemoresistance and tumor angiogenesis3.

Gene ID: 13846

NCBI Accession: NM_004444

UniProt: P54761

Pathways: RTK Signaling