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|Antigen||EPH Receptor B4 (EPHB4) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This EPH Receptor B4 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-EPH Receptor B4 AntibodyTarget Details EPH Receptor B4 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of EphB4 protein. (region surrounding Glu601)|
|Cross-Reactivity (Details)||Species reactivity (tested):Human and Mouse.|
|Purification||Affinity Chromatography using epitope-specific immunogen|
Target Details EPH Receptor B4Product Details anti-EPH Receptor B4 Antibody Application Details Handling Images back to top
|Alternative Name||EPHB4 (EPHB4 Antibody Abstract)|
|Background||EphB4, also known as Htk, Myk1, Tyro11, and Mdk2, is a member of the Eph receptor family, which binds of the ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains juxtamembrane motif with two tyrosines, which are the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxyl terminus, which includes one conserved tyrosine. Ligand recognition and binding leads to activation of intrinsic kinase activity. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands can bind the receptor, but do not induce receptor autophosphorylation and activation. The Eph receptors and ephrin ligands display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands.Ephs and ephrins play a significant role in angiogenesis.Synonyms: Ephrin type-B receptor 4, HTK, TYRO11, Tyrosine-protein kinase TYRO11, Tyrosine-protein kinase receptor HTK|
|Molecular Weight||approx. 108.0 kDa|
Application DetailsProduct Details anti-EPH Receptor B4 Antibody Target Details EPH Receptor B4 Handling Images back to top
Western Blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EPH Receptor B4 Antibody Target Details EPH Receptor B4 Application Details Images back to top
|Buffer||PBS, pH ~7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-EPH Receptor B4 Antibody Target Details EPH Receptor B4 Application Details Handling back to top