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|Antigen||Fibroblast Growth Factor 10 (FGF10) Antibodies|
|Conjugate||This FGF10 antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Product Details anti-FGF10 AntibodyTarget Details FGF10 Application Details Handling Images
E.coli derived recombinant Human FGF-10.
Type of Immunogen: Cell extract
Target Details FGF10Product Details anti-FGF10 Antibody Application Details Handling Images back to top
|Alternative Name||FGF10 (FGF10 Antibody Abstract)|
Name/Gene ID: FGF10
Synonyms: FGF10, Fibroblast growth factor 10, FGF-10, Keratinocyte growth factor 2
|Research Area||Cancer, Growth Factors, Angiogenesis|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Tube Formation, Positive Regulation of Response to DNA Damage Stimulus|
Application DetailsProduct Details anti-FGF10 Antibody Target Details FGF10 Handling Images back to top
Approved: ELISA (0.5 - 2 μg/mL), IHC, WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1 μg/mL-2.5 μg/mL with an overnight iincubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hFGF-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-10 (LS-C104659) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-10. Western Blot: To detect hFGF-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-10 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FGF10 Antibody Target Details FGF10 Application Details Images back to top
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ImagesProduct Details anti-FGF10 Antibody Target Details FGF10 Application Details Handling back to top