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Human FGF19 ELISA Kit

Recommended FGF19 Product (supplied by: Log in to see )

Antigen
Fibroblast Growth Factor 19 (FGF19) ELISA Kits
  • FGF-19
  • Fgf15
  • FGF19
Epitope
AA 25-216
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
17
5
2
2
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1889347
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32670116 ABIN418604 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32670116 ABIN924847 Sandwich ELISA Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Log in to see
0.32670116 ABIN1114912 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32670116 ABIN2708205 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012101001 ABIN3198530 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.0012101001 ABIN1569803 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012101001 ABIN838955 Log in to see
0.0012101001 ABIN2703009 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine 30-8000 pg/mL 30 pg/mL Log in to see
0.0012101001 ABIN2113899 Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Log in to see
0.0012101001 ABIN2745451 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012101001 ABIN2684255 Sandwich ELISA Cell Lysate, Plasma, Serum Log in to see
0.0012101001 ABIN2533387 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
0.0012101001 ABIN2082970 Sandwich ELISA Log in to see
0.0012101001 ABIN2947481 15.625-1000 pg/mL 15.625 pg/mL Log in to see

Similar FGF19 ELISA Kits

Application / Reactivity Human
ELISA 15 ELISA Kits

General

Antigen Fibroblast Growth Factor 19 (FGF19) ELISA Kits
Epitope AA 25-216
Reactivity Human
Kits with alternative reactivity to:
(17), (5), (2), (2), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Supplier Log in to see

Product Details FGF19 ELISA Kit

Target details Application Details Handling Images
Purpose For quantitative detection of human FGF19 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human FGF19
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human FGF19 antibod - 1
  • Lyophilized recombinant human FGF19 standar - 10ng/tubex2
  • Biotinylated anti- human FGF19 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: L25-K216

Target details

Product Details FGF19 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name FGF19 (FGF19 ELISA Kit Abstract)
Background FGF19, Fibroblast growth factor 19, is a protein that in humans is encoded by the FGF19 gene. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. The FGF19 gene is mapped to 11q13.3. The deduced 216-amino acid FGF19 protein contains asignal sequence and 2 cysteine residues that are conserved in the FGF family. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. FGF19 stimulates hepatic protein and glycogen synthesis but does not induce lipogenesis. The effects of FGF19 are independent of the activity of either insulin or the protein kinase Akt and, instead, are mediated through a mitogen-activated protein kinase signaling pathway that activates components of the protein translation machinery and stimulates glycogen synthase activity.
Gene ID 9965
UniProt O95750
Research Area Cancer, Growth Factors, Wnt
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details FGF19 ELISA Kit Target details Handling Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human FGF19 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF19 has been precoated onto 96-well plates. Standards(E.coli, L25-K216) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF19 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FGF19 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human FGF19 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human FGF19 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 103, Standard deviation: 6.69, CV(%): 6.5
  • Sample 2: n=16, Mean(pg/ml): 396, Standard deviation: 17, CV(%): 4.3
  • Sample 3: n=16, Mean(pg/ml): 669, Standard deviation: 25.4, CV(%): 3.8,
  • Sample 1: n=24, Mean(pg/ml): 152, Standard deviation: 10.94, CV(%): 7.2
  • Sample 2: n=24, Mean(pg/ml): 388, Standard deviation: 20.95, CV(%): 5.4
  • Sample 3: n=24, Mean(pg/ml): 709, Standard deviation: 34.74, CV(%): 4.9
Restrictions For Research Use only

Handling

Product Details FGF19 ELISA Kit Target details Application Details Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

Images

Product Details FGF19 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for FGF19 ELISA Kit (Fibroblast Growth Factor 19) (ABIN1889347) Human FGF19 PicoKine ELISA Kit standard curve