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anti-Human FGF2 antibody for Functional Studies

Recommended FGF2 Antibody (supplied by: Log in to see )

Antigen
Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies
  • bFGF
  • BFGF
  • Fgf-2
  • FGF-2
  • Fgfb
  • FGFB
  • HBGF-2
Reactivity
Human
255
55
40
18
16
9
5
3
1
1
1
Host
Rabbit
175
107
6
6
1
1
Clonality
Polyclonal
Conjugate
This FGF2 antibody is un-conjugated
28
16
11
6
5
5
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
194
167
91
33
25
21
19
16
14
11
11
10
6
4
3
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN116009
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008725739 ABIN116010 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
0.0008725739 ABIN115676 Func WB Rabbit Log in to see Polyclonal
0.0008725739 ABIN115677 Func WB Rabbit Log in to see Polyclonal
0.0008725739 ABIN187643 Func RIA Mouse IgG1 Log in to see BFM-1 2

Top referenced anti-FGF2 antibody for Functional Studies

  • Cow (Bovine) Monoclonal FGF2 Primary Antibody for Func, RIA - ABIN187643 (2 Publications): Matsuzaki, Yoshitake, Matuo, Sasaki, Nishikawa: Monoclonal antibodies against heparin-binding growth factor II/basic fibroblast growth factor that block its biological activity: invalidity of the antibodies for tumor angiogenesis. in Proceedings of the National Academy of Sciences of the United States of America 1990 (PubMed)

Similar anti-FGF2 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 156 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunodiffusion (ID) 2 Antibodies
Immunofluorescence (IF) 19 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 79 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies

General

Antigen Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies
Reactivity Human
(255), (55), (40), (18), (16), (9), (5), (3), (1), (1), (1)
Host Rabbit
(175), (107), (6), (6), (1), (1)
Clonality
Polyclonal
Conjugate This FGF2 antibody is un-conjugated
(28), (16), (11), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(194), (167), (91), (33), (25), (21), (19), (16), (14), (11), (11), (10), (6), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-FGF2 Antibody

Target Details FGF2 Application Details Handling Images
Specificity Human Fibroblast Growth Factor basic (FGF-2).
Purification Affinity chromatography.
Immunogen recombinant human FGF-2 (hFGF-2)

Target Details FGF2

Product Details anti-FGF2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name FGF Basic / FGF2 (FGF2 Antibody Abstract)
Background FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.Synonyms: BFGF, FGFB, Fibroblast growth factor 2 (basic), HBGF-2, HBGF2, Heparin-binding growth factor 2
Gene ID 2247
NCBI Accession NP_001997
UniProt P09038
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-FGF2 Antibody Target Details FGF2 Handling Images back to top
Application Notes Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhFGF-2 (0.3 ng/mL), a concentration of 0.25-0.40 μg/mL of this antibody is required. ELISA: (Indirect): To detect hFGF-2 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hFGF-2. (Sandwich): To detect hFGF-2 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human FGF-2 (PP1007B1, PP1007B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-2. Western Blot: To detect hFGF-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFGF-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-FGF2 Antibody Target Details FGF2 Application Details Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Centrifuge vial prior to opening!
Storage 4 °C
Storage Comment Store the antibody at 2-8 °C. Do not freeze!