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Human FGF21 ELISA Kit

Recommended FGF21 Product (supplied by: Log in to see )

Antigen
Fibroblast Growth Factor 21 (FGF21) ELISA Kits
  • FGF21
Epitope
AA 29-209
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
21
12
9
7
3
2
2
2
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1672889
$ 333.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32334948 ABIN418605 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.32334948 ABIN1114914 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.32334948 ABIN1305174 Sandwich ELISA Plasma, Serum 1.7-2000 pg/mL 1.7 pg/mL Log in to see
0.32334948 ABIN2708207 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012145225 ABIN612700 Sandwich ELISA Plasma, Cell Culture Supernatant 0.03 ng/mL Log in to see 7
0.0012145225 ABIN3198429 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012145225 ABIN2684258 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.31-2.00 ng/mL 0.31 ng/mL Log in to see 1
0.0012145225 ABIN1569810 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012145225 ABIN1053691 Serum, Cell Culture Supernatant, Body Fluids, Tissue Homogenate, Plasma Log in to see
0.0012145225 ABIN2703011 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 8-2.000 pg/mL 8 pg/mL Log in to see
0.0012145225 ABIN2042462 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012145225 ABIN2745452 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 30-1920 pg/mL 30 pg/mL Log in to see
0.0012145225 ABIN579452 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012145225 ABIN2533403 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.0012145225 ABIN2947484 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012145225 ABIN2082978 Sandwich ELISA Log in to see
0.0012145225 ABIN2112409 Competition ELISA Log in to see
0.0012145225 ABIN2871405 Sandwich ELISA Log in to see

Top referenced FGF21 ELISA Kit for ELISA

  • Human FGF21 ELISA Kit for Sandwich ELISA - ABIN612700 (7 Publications): Nishimura, Nakatake, Konishi, Itoh: Identification of a novel FGF, FGF-21, preferentially expressed in the liver. in Biochimica et biophysica acta 2000 (PubMed)

  • Human FGF21 ELISA Kit for Sandwich ELISA - ABIN2684258 (1 Publications): Suzuki, Lee, Vázquez-Medina, Viscarra, Crocker, Ortiz: Plasma FGF21 concentrations, adipose fibroblast growth factor receptor-1 and β-klotho expression decrease with fasting in northern elephant seals. in General and comparative endocrinology 2015 (PubMed)

  • Human FGF21 ELISA Kit for Sandwich ELISA - ABIN1672889 (2 Publications): Kharitonenkov, Shiyanova, Koester, Ford, Micanovic, Galbreath, Sandusky, Hammond, Moyers, Owens, Gromada, Brozinick, Hawkins, Wroblewski, Li, Mehrbod, Jaskunas, Shanafelt: FGF-21 as a novel metabolic regulator. in The Journal of clinical investigation 2005 (PubMed)

Similar FGF21 ELISA Kits

Application / Reactivity Human
ELISA 19 ELISA Kits

General

Antigen Fibroblast Growth Factor 21 (FGF21) ELISA Kits
Epitope AA 29-209
Reactivity Human
Kits with alternative reactivity to:
(21), (12), (9), (7), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details FGF21 ELISA Kit

Target details Application Details Handling References for FGF21 Kit (ABIN1672889) Images
Purpose For quantitative detection of human FGF21 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human FGF21
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human FGF21 antibod - 1
  • Lyophilized recombinant human FGF21 standar - 10ng/tubex2
  • Biotinylated anti- human FGF21 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: H29-S209

Target details

Product Details FGF21 ELISA Kit Application Details Handling References for FGF21 Kit (ABIN1672889) Images back to top
Antigen
Alternative Name FGF21 (FGF21 ELISA Kit Abstract)
Background Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al.(2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.
Gene ID 26291
UniProt Q9NSA1
Research Area Cancer, Growth Factors, Angiogenesis
Pathways RTK Signaling

Application Details

Product Details FGF21 ELISA Kit Target details Handling References for FGF21 Kit (ABIN1672889) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human FGF21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF21 has been precoated onto 96-well plates. Standards(E.coli, H29-S209) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FGF21 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human FGF21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human FGF21 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 256, Standard deviation: 11.52, CV(%): 4.5
  • Sample 2: n=16, Mean(pg/ml): 645, Standard deviation: 21.93, CV(%): 3.4
  • Sample 3: n=16, Mean(pg/ml): 1478, Standard deviation: 69.47, CV(%): 4.7,
  • Sample 1: n=24, Mean(pg/ml): 247, Standard deviation: 24.45, CV(%): 9.9
  • Sample 2: n=24, Mean(pg/ml): 649, Standard deviation: 48.03, CV(%): 7.4
  • Sample 3: n=24, Mean(pg/ml): 1426, Standard deviation: 89.84, CV(%): 6.3
Restrictions For Research Use only

Handling

Product Details FGF21 ELISA Kit Target details Application Details References for FGF21 Kit (ABIN1672889) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for FGF21 Kit (ABIN1672889)

Product Details FGF21 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Kharitonenkov, Shiyanova, Koester, Ford, Micanovic, Galbreath, Sandusky, Hammond, Moyers, Owens, Gromada, Brozinick, Hawkins, Wroblewski, Li, Mehrbod, Jaskunas, Shanafelt: "FGF-21 as a novel metabolic regulator." in: The Journal of clinical investigation, Vol. 115, Issue 6, pp. 1627-35, 2005

Nishimura, Nakatake, Konishi, Itoh: "Identification of a novel FGF, FGF-21, preferentially expressed in the liver." in: Biochimica et biophysica acta, Vol. 1492, Issue 1, pp. 203-6, 2000

Images

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Supplier Images
ELISA image for FGF21 ELISA Kit (Fibroblast Growth Factor 21) (ABIN1672889) Human FGF21 PicoKine ELISA Kit standard curve