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Mouse (Murine) FGF21 ELISA Kit

Recommended FGF21 Product (supplied by: Log in to see )

Antigen
Fibroblast Growth Factor 21 (FGF21) ELISA Kits
  • FGF21
Epitope
AA 29-210
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
22
11
9
7
3
2
2
2
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
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Catalog No. ABIN2859244
$ 333.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32748017 ABIN1114913 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.32748017 ABIN2761940 Competition ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 6.17-500 pg/mL 6.17 pg/mL Log in to see 1
0.0012092679 ABIN838959 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012092679 ABIN1569811 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.813-500 pg/mL 7.813 pg/mL Log in to see
0.0012092679 ABIN2748808 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012092679 ABIN579453 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012092679 ABIN2043473 Sandwich ELISA 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.0012092679 ABIN2947485 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012092679 ABIN2947488 6.17-500 pg/mL 6.17 pg/mL Log in to see

Top referenced FGF21 ELISA Kit for ELISA

Similar FGF21 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 10 ELISA Kits

General

Antigen Fibroblast Growth Factor 21 (FGF21) ELISA Kits
Epitope AA 29-210
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(22), (11), (9), (7), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 1 reference available
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Product Details FGF21 ELISA Kit

Target details Application Details Handling References for FGF21 Kit (ABIN2859244) Images
Purpose For quantitative detection of mouse FGF21 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse FGF21
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- Mouse FGF21 antibod - 1
  • Lyophilized recombinant Mouse FGF21 standar - 10ng/tubex2
  • Biotinylated anti- Mouse FGF21 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Immunogen Expression system for standard: E.coli
Immunogen sequence: A29-S210

Target details

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Antigen
Alternative Name FGF21 (FGF21 ELISA Kit Abstract)
Background Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.
Gene ID 56636
UniProt Q9JJN1
Research Area Cancer, Growth Factors, Angiogenesis
Pathways RTK Signaling

Application Details

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Application Notes Optimal working dilution should be determined by the investigator.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse FGF21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FGF21 has been precoated onto 96-well plates. Standards(E.coli,A29-S210) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FGF21 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse FGF21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse FGF21 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 245, Standard deviation: 11.51, CV(%): 4.7
  • Sample 2: n=16, Mean(pg/ml): 656, Standard deviation: 28.20, CV(%): 4.3
  • Sample 3: n=16, Mean(pg/ml): 1422, Standard deviation: 71.1, CV(%): 5.0,
  • Sample 1: n=24, Mean(pg/ml): 210, Standard deviation: 12.81, CV(%): 6.1
  • Sample 2: n=24, Mean(pg/ml): 670, Standard deviation: 43.55, CV(%): 6.5
  • Sample 3: n=24, Mean(pg/ml): 1572, Standard deviation: 99.03, CV(%): 6.3
Restrictions For Research Use only

Handling

Product Details FGF21 ELISA Kit Target details Application Details References for FGF21 Kit (ABIN2859244) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for FGF21 Kit (ABIN2859244)

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Product cited in:

Liu, Pan, Chen, Yang, Zhao, Jia, Zhu, Zhang, Gao, Li, Han, Duan: "Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy." in: Scientific reports, Vol. 5, pp. 11219, 2015

Images

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Supplier Images
ELISA image for FGF21 ELISA Kit (Fibroblast Growth Factor 21) (ABIN2859244) Mouse FGF21 PicoKine ELISA Kit standard curve