Mouse (Murine) FGF21 ELISA Kit

Recommended FGF21 Product (supplied by: Log in to see )

Antigen
Fibroblast Growth Factor 21 (FGF21) ELISA Kits
  • FGF21
  • fibroblast growth factor 21
  • FGF21
  • fgf21
  • Fgf21
  • LOC100356340
Epitope
AA 29-210
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
26
19
14
12
3
2
2
2
2
2
1
1
1
Method Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
Options
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Catalog No. ABIN2859244
$ 333.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.702948 ABIN2761940 Competition ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 12.5-200 pg/mL 12.5 pg/mL Log in to see 1
9.702948 ABIN838959 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate 31.25-2000 pg/mL 31.25 pg/mL Log in to see
9.702948 ABIN1114913 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
9.702948 ABIN4947578 Competition ELISA Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate, Biological Fluids 1.23-100 pg/mL 1.23 pg/mL Log in to see
1 ABIN1081421 Sandwich ELISA Serum, Plasma, Biological Fluids 1.37-1.000 pg/mL 1.37 pg/mL Log in to see 1
1 ABIN1569811 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Log in to see
1 ABIN5592039 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN4947577 Competition ELISA Log in to see
1 ABIN2748808 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN5520534 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 7.8-500 pg/mL 7.8 pg/mL Log in to see
1 ABIN512691 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
1 ABIN4969110 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Log in to see
1 ABIN579453 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN2043473 Sandwich ELISA 7.81-500 pg/mL 7.81 pg/mL Log in to see
1 ABIN5068388 Sandwich ELISA Log in to see
1 ABIN2947485 31.25-2000 pg/mL 31.25 pg/mL Log in to see
1 ABIN2947488 6.17-500 pg/mL 6.17 pg/mL Log in to see
1 ABIN2944127 1.37-1000 pg/mL 1.37 pg/mL Log in to see
1 ABIN5072175 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

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General

Antigen Fibroblast Growth Factor 21 (FGF21) ELISA Kits
Epitope AA 29-210
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(26), (19), (14), (12), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details FGF21 ELISA Kit

Target details Application Details Handling ProductDetails: References for FGF21 Kit (ABIN2859244) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse FGF21
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Analytical Method Quantitative
Specificity E.coli, A29-S210
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: A29-S210

Target details

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Antigen
Alternative Name FGF21 (FGF21 ELISA Kit Abstract)
Background

Protein Function: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity probably requires the presence of KLB. .

Background: Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.

Synonyms: Fibroblast growth factor 21,FGF-21,Fgf21,

Full Gene Name: Fibroblast growth factor 21

Cellular Localisation: Secreted.
Gene ID 56636
UniProt Q9JJN1
Research Area Cancer, Growth Factors, Angiogenesis
Pathways RTK Signaling

Application Details

Product Details FGF21 ELISA Kit Target details Handling ProductDetails: References for FGF21 Kit (ABIN2859244) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Tissue Specificity: Most abundantly expressed in the liver, also expressed in the thymus at lower levels. .

Plate Pre-coated
Protocol mouse FGF21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FGF21 has been precoated onto 96-well plates. Standards(E.coli,A29-S210) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FGF21 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse FGF21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse FGF21 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 245, Standard deviation: 11.51, CV(%): 4.7
  • Sample 2: n=16, Mean(pg/ml): 656, Standard deviation: 28.20, CV(%): 4.3
  • Sample 3: n=16, Mean(pg/ml): 1422, Standard deviation: 71.1, CV(%): 5.0,
  • Sample 1: n=24, Mean(pg/ml): 210, Standard deviation: 12.81, CV(%): 6.1
  • Sample 2: n=24, Mean(pg/ml): 670, Standard deviation: 43.55, CV(%): 6.5
  • Sample 3: n=24, Mean(pg/ml): 1572, Standard deviation: 99.03, CV(%): 6.3
Restrictions For Research Use only

Handling

Product Details FGF21 ELISA Kit Target details Application Details ProductDetails: References for FGF21 Kit (ABIN2859244) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

ProductDetails: References for FGF21 Kit (ABIN2859244)

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Product cited in:

Liu, Pan, Chen, Yang, Zhao, Jia, Zhu, Zhang, Gao, Li, Han, Duan: "Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy." in: Scientific reports, Vol. 5, pp. 11219, 2015

Images

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Supplier Images
ELISA image for FGF21 ELISA Kit (Fibroblast Growth Factor 21) (ABIN2859244) Mouse FGF21 PicoKine ELISA Kit standard curve