We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Human|
|Cell Culture (CC)||1 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Cytometry by Time of Flight (CyTOF)||14 Antibodies|
|Dot Blot (DB)||185 Antibodies|
|ELISA (Capture)||3 Antibodies|
|ELISA (Detection)||1 Antibodies|
|Enzyme Immunoassay (EIA)||19 Antibodies|
|Flow Cytometry (FACS)||292 Antibodies|
|Functional Studies (Func)||5 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunochromatography (IC)||5 Antibodies|
|Immunocytochemistry (ICC)||89 Antibodies|
|Immunoelectron Microscopy (IEM)||6 Antibodies|
|Immunofluorescence (IF)||297 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||8 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||102 Antibodies|
|Immunohistochemistry (IHC)||308 Antibodies|
|Antigen||V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies|
|Epitope||Extracellular Domain Alternatives|
|Conjugate||This HER2 antibody is un-conjugated Alternatives|
Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
|3 references available|
|Supplier||Log in to see|
Product Details anti-HER2 AntibodyTarget Details HER2 Application Details Handling ProductDetails: References for anti-HER2 antibody (ABIN180510) Images
|Specificity||This antibody detects c-erbB-2 / Her-2 / neu. It reacts with the intact cells( 1,2), shows moderate effect on c-erbB-2 internalization (1), slightly increases (~2-fold) the tyrosine kinase activity (1), and inhibits (~90 %) the tumorigenic growth in CD1/nude mice of N87 human gastric carcinoma cell line overexpressing c-erbB-2 (1,2,3). Does not react with Mouse and rat.|
|Characteristics||Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19, Receptor tyrosine-protein kinase erbB-2,NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2|
|Purification||Protein G chromatography|
|Immunogen||Intact SKBR-3 breast cancer cells (1)Remarks: Epitope: Extracellular domain. Mol. Wt. of Antigen: 185 kDa.|
Target Details HER2Product Details anti-HER2 Antibody Application Details Handling ProductDetails: References for anti-HER2 antibody (ABIN180510) Images back to top
|Alternative Name||CD340 / ERBB2 / HER2 (ERBB2 Antibody Abstract)|
|Background||C-erbB-2, second member (c-erbB-2/HER-2/neu) of the c-erbB family is a receptor tyrosine kinase. It exhibits extracellular domains with two cysteine-rich sequences, and a cytoplasmic tyrosine kinase domain flanked by large hydrophilic tails that carry several tyrosine autophosphorylation sites. Approximately 25 % of primary breast and ovarian tumors were found to overexpress the protein.Synonyms: HER-2, MNL19, NEU, NEU proto-oncogene, NGL, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, c-erbB-2, p185erbB2|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development|
Application DetailsProduct Details anti-HER2 Antibody Target Details HER2 Handling ProductDetails: References for anti-HER2 antibody (ABIN180510) Images back to top
Inhibits Tumor Growth (approx. 90 % ) (1,2,3). Inhibits Cell Proliferation (1). Effect on c-erbB-2 Internalization (Moderate) (1,3). Electron Microscopy (2). Immunofluorescence. Immunohistology (Frozen only). Does not work in Immunoprecipitation. Positive Control: SKBR-3 cells, or breast carcinomas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-HER2 Antibody Target Details HER2 Application Details ProductDetails: References for anti-HER2 antibody (ABIN180510) Images back to top
|Buffer||10 mM PBS, pH 7.4|
|Storage||4 °C/-20 °C|
Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ProductDetails: References for anti-HER2 antibody (ABIN180510)Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Klapper, Vaisman, Hurwitz, Pinkas-Kramarski, Yarden, Sela: "A subclass of tumor-inhibitory monoclonal antibodies to ErbB-2/HER2 blocks crosstalk with growth factor receptors." in: Oncogene, Vol. 14, Issue 17, pp. 2099-109, 1997
Hurwitz, Stancovski, Sela, Yarden: "Suppression and promotion of tumor growth by monoclonal antibodies to ErbB-2 differentially correlate with cellular uptake." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 8, pp. 3353-7, 1995
Stancovski, Hurwitz, Leitner, Ullrich, Yarden, Sela: "Mechanistic aspects of the opposing effects of monoclonal antibodies to the ERBB2 receptor on tumor growth." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 19, pp. 8691-5, 1991