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anti-Human HER2 antibody for Immunoprecipitation

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • c-erbB2
  • c-neu
  • CD340
  • Erbb-2
  • HER-2
  • HER-2/neu
  • HER2
  • mKIAA3023
  • MLN 19
  • NEU
  • Neu
  • NGL
  • TKR1
Epitope
C-Term, pTyr1248
119
82
64
54
54
53
51
44
37
36
32
29
21
20
20
20
20
19
19
19
19
19
19
18
18
18
15
14
14
14
13
13
13
12
10
10
9
8
8
8
8
7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
1322
418
260
24
13
4
3
3
3
3
2
2
1
1
1
Host
Mouse
969
495
25
18
5
5
Clonality
Monoclonal
Conjugate
This HER2 antibody is un-conjugated
74
65
60
52
45
41
17
17
10
10
9
8
8
8
8
8
8
7
7
7
7
7
7
7
7
7
7
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
680
574
331
323
301
281
234
173
104
90
86
21
17
6
6
5
5
5
3
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN180514
$ 716.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089556724 ABIN493356 IP WB Rabbit C-Term, pTyr1129 Log in to see Polyclonal 3
0.00089556724 ABIN967787 IF IP IHC WB Mouse IgG2b AA 182-373 Log in to see 42-c-erbB 5
0.00089556724 ABIN967786 IF IP IHC WB Mouse IgG2b AA 182-373 Log in to see 42-c-erbB 5
0.00089556724 ABIN967459 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 1242-1255 Log in to see 3B5 9
0.00089556724 ABIN181400 IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 1
0.00089556724 ABIN180513 IHC (p) IP WB Mouse IgG1 C-Term Log in to see e2-4001-3B5
0.00089556724 ABIN493049 IP WB Rabbit Log in to see Polyclonal 3
0.00089556724 ABIN151232 IHC (p) IP IHC WB Rabbit AA 975-1025 Log in to see Polyclonal 1
0.00089556724 ABIN372478 IHC (fro) IHC (p) IP WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal
0.00089556724 ABIN305740 IHC (fro) IHC (p) IP WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal
0.00089556724 ABIN119013 FACS IHC (fro) IHC (p) IP WB Rat IgG2a Log in to see ICR55
0.00089556724 ABIN318038 EIA IHC (p) IP WB Rabbit pTyr877 Log in to see Polyclonal
0.00089556724 ABIN959522 IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see 3B5
0.00089556724 ABIN318035 IHC (p) IP WB Rabbit pTyr1221, pTyr1222 Log in to see Polyclonal
0.00089556724 ABIN180512 Func IP Mouse IgG1 Log in to see N28 3
0.00089556724 ABIN2661687 Func ICC FACS IF ICFC IP WB FITC Mouse IgG1 kappa Log in to see 24D2 6
0.00089556724 ABIN2704879 IP IHC WB Rabbit C-Term Log in to see Polyclonal
0.00089556724 ABIN2704878 IP IHC WB Rabbit C-Term, pTyr1221, pTyr1222 Log in to see Polyclonal
0.00089556724 ABIN385644 IHC (p) IP WB Mouse IgG1 Cytoplasmic Domain Log in to see e2-4001
0.00089556724 ABIN2888431 IHC (p) IP ELISA WB Rabbit IgG pTyr1221 Log in to see Polyclonal

Top referenced anti-HER2 antibody for Immunoprecipitation

Similar anti-HER2 Antibodies

Application / Reactivity Rat (Rattus) Human
Dot Blot (DB) 19 Antibodies 192 Antibodies
ELISA 71 Antibodies 439 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies 17 Antibodies
Flow Cytometry (FACS) 27 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 33 Antibodies
Immunofluorescence (IF) 74 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 89 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 23 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 47 Antibodies
Immunoprecipitation (IP) 47 Antibodies
Western Blotting (WB) 166 Antibodies
Cellular Assay (CA) 1 Antibodies
ELISA (Capture) 1 Antibodies

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope C-Term, pTyr1248
(119), (82), (64), (54), (54), (53), (51), (44), (37), (36), (32), (29), (21), (20), (20), (20), (20), (19), (19), (19), (19), (19), (19), (18), (18), (18), (15), (14), (14), (14), (13), (13), (13), (12), (10), (10), (9), (8), (8), (8), (8), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(1322), (418), (260), (24), (13), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(969), (495), (25), (18), (5), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HER2 antibody is un-conjugated
(74), (65), (60), (52), (45), (41), (17), (17), (10), (10), (9), (8), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (2), (2), (2)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(680), (574), (331), (323), (301), (281), (234), (173), (104), (90), (86), (21), (17), (6), (6), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 11 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN180514) Images
Specificity This is highly specific to the activated, tyrosine- hosphorylated (pTyr1248) form of c-erbB-2 and does not cross react with closely related receptors such as EGFR, HER-3, or HER-4 (1).
Characteristics Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19, Receptor tyrosine-protein kinase erbB-2,NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2
Purification Purified
Immunogen Tyrosine-phosphorylated synthetic peptide corresponding to the C-terminus of humanc-erbB-2 protein (P-Tyr1248). AA Sequence: 1242-1255 with pTyr1248 [Lys- TAENPE-pY-LGLDVPV]Remarks: Mol. Wt. of Antigen: 185 kDa.
Clone PN2A
Isotype IgG1

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 Antibody (ABIN180514) Images back to top
Antigen
Alternative Name CD340 / ERBB2 / HER2 (ERBB2 Antibody Abstract)
Background C-erbB-2 (HER-2/neu) is a 185 kDa receptor tyrosine kinase belonging to the epidermal growth factor receptor family, which also includes EGFR (HER-1), c-erbB-3 (HER-3), and c-erbB-4 (HER-4). Amplification of the HER-2 gene with overexpression of the protein occurs in 20-30 % of breast cancers (5,8), and tumor progression in mammary cancer is associated with elevated levels of tyrosine-phosphorylated neu and erbB-3. Tyr1248 is the phosphorylation site that is most tightly linked to oncogenic transformation and coupling to the ras/MAP kinase signaling pathway (3). Cases of invasive breast carcinoma with the phosphorylated receptor have displayed aggressive clinicopathological features and adverse prognoses. Activation of HER-2 is frequent in ductal carcinoma in situ suggesting that HER-2 signaling plays a critical role in the early stages of breast tumorigenesis (8).Synonyms: HER-2, MNL19, NEU, NEU proto-oncogene, NGL, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, c-erbB-2, p185erbB2
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 Antibody (ABIN180514) Images back to top
Application Notes Flow Cytometry (Not verified). Immunofluorescence (1,4,10). Immunoprecipitation (Not verified)Western Blotting (1,9)Immunohistology (Formalin/paraffin) (1,2,3,5,8): Use antibody at 10-20 μg/mL for 2 hrs atRT. [Staining of formalin/paraffin tissues REQUIRES boiling tissue sections in 10 mM citratebuffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. ]. Positive Control: EGF-treated SKBR3 cells. About 5 % of breast carcinomas are positive forphospho-c-erbB-2/HER-2/neu oncoprotein.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 Antibody (ABIN180514) Images back to top
Concentration 0.2 mg/mL
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-HER2 Antibody (ABIN180514)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Background publications

Ramsauer, Carraway, Salas, Carraway: "Muc4/sialomucin complex, the intramembrane ErbB2 ligand, translocates ErbB2 to the apical surface in polarized epithelial cells." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 30142-7, 2003

Xu, Yuan, Jung, Yang, Basso, Rosen, Chung, Trepel, Neckers: "The heat shock protein 90 inhibitor geldanamycin and the ErbB inhibitor ZD1839 promote rapid PP1 phosphatase-dependent inactivation of AKT in ErbB2 overexpressing breast cancer cells." in: Cancer research, Vol. 63, Issue 22, pp. 7777-84, 2003

DiGiovanna, Chu, Davison, Howe, Carter, Claus, Stern: "Active signaling by HER-2/neu in a subpopulation of HER-2/neu-overexpressing ductal carcinoma in situ: clinicopathological correlates." in: Cancer research, Vol. 62, Issue 22, pp. 6667-73, 2002

Ouyang, Gulliford, Huang, Harper-Wynne, Shousha, Epstein: "Multisite phosphotyping of the ErbB-2 oncoprotein in human breast cancer." in: Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology, Vol. 6, Issue 1, pp. 17-25, 2001

Ouyang, Gulliford, Zhang, Smith, Huang, Epstein: "Association of ErbB2 Ser1113 phosphorylation with epidermal growth factor receptor co-expression and poor prognosis in human breast cancer." in: Molecular and cellular biochemistry, Vol. 218, Issue 1-2, pp. 47-54, 2001

Christensen, Schreck, Chan, Wang, Yang, Liu, Cui, Sun, Wei, Cherrington, Mendel: "High levels of HER-2 expression alter the ability of epidermal growth factor receptor (EGFR) family tyrosine kinase inhibitors to inhibit EGFR phosphorylation in vivo." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 7, Issue 12, pp. 4230-8, 2001

Thor, Liu, Edgerton, Moore, Kasowitz, Benz, Stern, DiGiovanna: "Activation (tyrosine phosphorylation) of ErbB-2 (HER-2/neu): a study of incidence and correlation with outcome in breast cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 18, Issue 18, pp. 3230-9, 2000

Brandt, Roetger, Dittmar, Nikolai, Seeling, Merschjann, Nofer, Dehmer-Möller, Junker, Assmann, Zaenker: "c-erbB-2/EGFR as dominant heterodimerization partners determine a motogenic phenotype in human breast cancer cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 13, Issue 14, pp. 1939-49, 1999

DiGiovanna, Lerman, Coffey, Muller, Cardiff, Stern: "Active signaling by Neu in transgenic mice." in: Oncogene, Vol. 17, Issue 14, pp. 1877-84, 1998

DiGiovanna, Carter, Flynn, Stern: "Functional assay for HER-2/neu demonstrates active signalling in a minority of HER-2/neu-overexpressing invasive human breast tumours." in: British journal of cancer, Vol. 74, Issue 5, pp. 802-6, 1996

DiGiovanna, Stern: "Activation state-specific monoclonal antibody detects tyrosine phosphorylated p185neu/erbB-2 in a subset of human breast tumors overexpressing this receptor." in: Cancer research, Vol. 55, Issue 9, pp. 1946-55, 1995

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN180514) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (C-Term) (ABIN180514) anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (C-Term), (pTyr1248) antibody