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Rat (Rattus) HGF ELISA Kit

Recommended HGF Product (supplied by: Log in to see )

Antigen
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) ELISA Kits
  • AI838057
  • c-Met
  • C230052L06Rik
  • DFNB39
  • F-TCF
  • HGF
  • HGF/SF
  • HGFB
  • HGFR
  • HPTA
  • NK1
  • NK2
  • Par4
  • SF
  • SF/HGF
Epitope
AA 33-495, AA 496-728
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
32
17
15
11
10
8
4
3
3
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
156-10.000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1889441
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32666144 ABIN416317 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see 6
0.32666144 ABIN1115406 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.32666144 ABIN2708645 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012121401 ABIN365554 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012121401 ABIN627165 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Log in to see
0.0012121401 ABIN1570290 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012121401 ABIN2748164 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids 0.8-200 ng/mL Log in to see
0.0012121401 ABIN510698 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012121401 ABIN2684468 Sandwich ELISA Plasma (EDTA), Plasma (citrate), Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012121401 ABIN579316 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012121401 ABIN2534434 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
0.0012121401 ABIN2086495 Sandwich ELISA Log in to see
0.0012121401 ABIN2948252 62.5-4000 pg/mL 62.5 pg/mL Log in to see

Top referenced HGF ELISA Kit for ELISA

Similar HGF ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 14 ELISA Kits

General

Antigen Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) ELISA Kits
Epitope AA 33-495, AA 496-728
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(32), (17), (15), (11), (10), (8), (4), (3), (3), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 156-10.000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 3 references available
Supplier Log in to see

Product Details HGF ELISA Kit

Target details Application Details Handling References for HGF Kit (ABIN1889441) Images
Purpose For quantitative detection of rat HGF in cell culture supernates, serum and plasma(EDTA, citrate).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate)
Detection Method Colorimetric
Specificity Natural and recombinant rat HGF
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- rat HGF antibod - 1
  • Lyophilized recombinant rat HGF standar - 10ng/tubex2
  • Biotinylated anti- rat HGF antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: Q33-R495(alpha) & V496-L728(beta)

Target details

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Antigen
Alternative Name HGF (HGF ELISA Kit Abstract)
Background Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000.1 The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus.2 HGF may serve as a paracrine mediator to control placental development and growth.3 This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin.4 The gene encoding the human HGF is assigned to human chromosome 7.
Gene ID 24446
UniProt P17945
Research Area Cancer, Angiogenesis, Hormones, Growth Factors
Pathways RTK Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol rat HGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HGF has been precoated onto 96-well plates. Standards(NSO, Q33-R495(α) & V496-L728(β)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat HGF amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat HGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat HGF standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 1765, Standard deviation: 74.13, CV(%): 4.2
  • Sample 2: n=16, Mean(pg/ml): 3081, Standard deviation: 141.7, CV(%): 4.6
  • Sample 3: n=16, Mean(pg/ml): 6143, Standard deviation: 344, CV(%): 5.6,
  • Sample 1: n=24, Mean(pg/ml): 1860, Standard deviation: 126.5, CV(%): 6.8
  • Sample 2: n=24, Mean(pg/ml): 3542, Standard deviation: 180.6, CV(%): 5.1
  • Sample 3: n=24, Mean(pg/ml): 6496, Standard deviation: 487.2, CV(%): 7.5
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for HGF Kit (ABIN1889441)

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Product cited in:

Secchiero, Corallini, Zavan, Tripodo, Vindigni, Zauli: "Mesenchymal stem cells display hepato-protective activity in lymphoma bearing xenografts." in: Investigational new drugs, Vol. 30, Issue 2, pp. 803-7, 2012

Background publications

Kilby, Afford, Li, Strain, Ahmed, Whittle: "Localisation of hepatocyte growth factor and its receptor (c-met) protein and mRNA in human term placenta." in: Growth factors (Chur, Switzerland), Vol. 13, Issue 1-2, pp. 133-9, 1996

Rubin, Chan, Bottaro, Burgess, Taylor, Cech, Hirschfield, Wong, Miki, Finch: "A broad-spectrum human lung fibroblast-derived mitogen is a variant of hepatocyte growth factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 2, pp. 415-9, 1991

Images

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Supplier Images
ELISA image for HGF ELISA Kit (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor)) (ABIN1889441) Rat HGF PicoKine ELISA Kit standard curve