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HGF ELISA Kit
HGF
Reactivity: Human
Colorimetric
Sandwich ELISA
3-2000 pg/mL
Cell Lysate, Tissue Lysate
Product Details HGF ELISA Kit
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Target
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HGF
(Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
Reactivity
All reactivities for HGF elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
3-2000 pg/mL
Minimum Detection Limit
3 pg/mL
Application
ELISA
Purpose
Human HGF ELISA Kit for cell and tissue lysate samples.
Sample Type
Tissue Lysate, Cell Lysate
Analytical Method
Quantitative
Specificity
The antibody pair provided in this kit recognizes human HGF.
Sensitivity
3 pg/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis Cell lysate buffer
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for HGF
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Target
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HGF
(Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
Alternative Name
HGF (HGF Products )
Synonyms
DFNB39 ELISA Kit, F-TCF ELISA Kit, HGFB ELISA Kit, HPTA ELISA Kit, SF ELISA Kit, HGF ELISA Kit, C230052L06Rik ELISA Kit, HGF/SF ELISA Kit, NK1 ELISA Kit, NK2 ELISA Kit, SF/HGF ELISA Kit, hepatocyte growth factor ELISA Kit, HGF ELISA Kit, Hgf ELISA Kit
Background
Gene Names: HGF HPTA Protein names: Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain]
Gene ID
3082
UniProt
P14210
Pathways
RTK Signaling , Carbohydrate Homeostasis , Glycosaminoglycan Metabolic Process , Synaptic Membrane , Signaling of Hepatocyte Growth Factor Receptor
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