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|Antigen||Insulin-Like Growth Factor 1 Receptor (IGF1R) Antibodies|
|Epitope||Chain alpha Alternatives|
|Conjugate||This IGF1R antibody is un-conjugated Alternatives|
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|4 references available|
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Product Details anti-IGF1R AntibodyTarget Details IGF1R Application Details Handling References for anti-IGF1R antibody (ABIN111141) Images
|Specificity||Clone 1H7 recognizes an epitope in the alpha subunits of CD221, demonstrated by Western blotting (Ref.1). Clone 1H7 is reported to partically block binding of IGF-I and IGF-II to CD221 (Ref.1)|
|Purification||Affinity Chromatography on Protein G|
|Endotoxin Level||Low Endotoxin|
Target Details IGF1RProduct Details anti-IGF1R Antibody Application Details Handling References for anti-IGF1R antibody (ABIN111141) Images back to top
|Alternative Name||CD221 / IGF1R (IGF1R Antibody Abstract)|
|Background||CD221, a 155 kD tyrosine kinase, is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins.Synonyms: IGF-I receptor, IGF1 Receptor, Insulin-like growth factor 1 receptor|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
|Pathways||RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy|
Application DetailsProduct Details anti-IGF1R Antibody Target Details IGF1R Handling References for anti-IGF1R antibody (ABIN111141) Images back to top
Functional Assays. Western blotting. Immunohistochemistry on Paraffin Sections: 1/10-1/40. This product does not requireprotein digestion pre-treatment of paraffin sections. Use pancreas or placenta as histologypositive control tissue. Flow Cytometry Use 10 μL of 1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IGF1R Antibody Target Details IGF1R Application Details References for anti-IGF1R antibody (ABIN111141) Images back to top
|Buffer||PBS without preservatives or stabilizers|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.|
References for anti-IGF1R antibody (ABIN111141)Product Details anti-IGF1R Antibody Target Details IGF1R Application Details Handling Images back to top
|Product cited in:||
Hantel, Lewrick, Reincke, Süss, Beuschlein: "Liposomal doxorubicin-based treatment in a preclinical model of adrenocortical carcinoma." in: The Journal of endocrinology, Vol. 213, Issue 2, pp. 155-61, 2012
Hantel, Lewrick, Schneider, Zwermann, Perren, Reincke, Süss, Beuschlein: "Anti insulin-like growth factor I receptor immunoliposomes: a single formulation combining two anticancer treatments with enhanced therapeutic efficiency." in: The Journal of clinical endocrinology and metabolism, Vol. 95, Issue 2, pp. 943-52, 2010
Beauvais, Rapraeger: "Syndecan-1 couples the insulin-like growth factor-1 receptor to inside-out integrin activation." in: Journal of cell science, Vol. 123, Issue Pt 21, pp. 3796-807, 2010
Li, Kato, Paz, Kasuya, Fujita-Yamaguchi: "Two new monoclonal antibodies against the alpha subunit of the human insulin-like growth factor-I receptor." in: Biochemical and biophysical research communications, Vol. 196, Issue 1, pp. 92-8, 1993
ImagesProduct Details anti-IGF1R Antibody Target Details IGF1R Application Details Handling References for anti-IGF1R antibody (ABIN111141) back to top