Rat (Rattus) KIT Ligand ELISA Kit

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Antigen
KIT Ligand (KITLG) ELISA Kits
  • Xkl-1
  • Xsl
  • Xsl-1
  • Xsl-2
  • steel
  • KITLG
  • SCF
  • kitl
  • kl-1
  • mgf
  • scf
  • FPH2
  • KL-1
  • Kitl
  • MGF
  • SF
  • SHEP7
  • Clo
  • Con
  • Gb
  • Kitlg
  • Mgf
  • SLF
  • Sl
  • blz
  • contrasted
  • 710-712
  • CSF
  • KITL
  • KIT ligand
  • kit ligand
  • kitlg
  • KITLG
  • Kitl
  • Kitlg
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
28
26
19
10
7
7
7
2
2
2
2
2
1
1
1
Method Type
Sandwich ELISA
Detection Range
12-3.000 pg/mL
Minimum Detection Limit
12 pg/mL
Application
ELISA
Options
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Catalog No. ABIN2748629
$ 556.60
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.76768 ABIN365576 Sandwich ELISA Log in to see 1
9.76768 ABIN431526 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
9.76768 ABIN924843 Sandwich ELISA Cell Lysate, Serum, Plasma 16-2000 pg/mL 16 pg/mL Log in to see
9.76768 ABIN2859277 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (EDTA) 31.2-2000 pg/mL 31.2 pg/mL Log in to see
9.76768 ABIN1117235 Sandwich ELISA Serum, Plasma, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN5563470 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 12.3-3000 pg/mL 12.3 pg/mL Log in to see
1 ABIN1572293 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN627327 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 1.0-25 ng/mL 1.0 ng/mL Log in to see
1 ABIN579379 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant, Biological Fluids 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN5521030 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN3071907 Sandwich ELISA Body Fluids, Plasma, Cell Culture Supernatant, Serum 31-2000 pg/mL 31 pg/mL Log in to see
1 ABIN3071910 Sandwich ELISA Body Fluids, Plasma, Cell Culture Supernatant, Serum 31-2000 pg/mL 31 pg/mL Log in to see
1 ABIN2114430 Cell Lysate, Serum, Plasma 16-2000 pg/mL 16 pg/mL Log in to see
1 ABIN4973025 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN2539203 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
1 ABIN2684796 Sandwich ELISA Cell Lysate, Plasma, Serum Log in to see
1 ABIN2951550 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN2536391 Tissue Homogenate, Cell Lysate, Biological Fluids Log in to see
1 ABIN5074029 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

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General

Antigen KIT Ligand (KITLG) ELISA Kits
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(28), (26), (19), (10), (7), (7), (7), (2), (2), (2), (2), (2), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 12-3.000 pg/mL
Minimum Detection Limit 12 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details KIT Ligand ELISA Kit

Target details Application Details Handling ProductDetails: References for KIT Ligand Kit (ABIN2748629) Images
Purpose Rat SCF ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat 4-1BB, Activin A, Adiponectin, B7-1, CD48, CTACK, Decorin, Eotaxin, EphA5, Erythropoietin, FGF-BP, Fit-3 Ligand, Galectin-1, Galectin-3, Gas 1, GFR alpha-1, gp130, IL-1 R6, IL-1 ra, IL-2 R alpha, IL-3, IL-7, IL-17F, IL-22, JAM-A, MIP-1 alpha, Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, P-Cadherin, Prolactin, RANTES, SCF, TIM-1, TIMP-2, TREM-1, TWEAK R.
Sensitivity 12 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details KIT Ligand ELISA Kit Application Details Handling ProductDetails: References for KIT Ligand Kit (ABIN2748629) Images back to top
Antigen
Alternative Name SCF (KITLG ELISA Kit Abstract)
Background Kit ligand (Hematopoietic growth factor KL) (Mast cell growth factor) (MGF) (Stem cell factor) (SCF) (c-Kit ligand)
Gene ID 60427
UniProt P21581
Research Area Embryogenesis, Embryonic stem cells, Innate Immunity, Cytokines
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details KIT Ligand ELISA Kit Target details Handling ProductDetails: References for KIT Ligand Kit (ABIN2748629) Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Assay Procedure Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details KIT Ligand ELISA Kit Target details Application Details ProductDetails: References for KIT Ligand Kit (ABIN2748629) Images back to top
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

ProductDetails: References for KIT Ligand Kit (ABIN2748629)

Product Details KIT Ligand ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Chao, Viets, Donham, Wood, Gonzalez: "Dexamethasone blocks the systemic inflammation of alveolar hypoxia at several sites in the inflammatory cascade." in: American journal of physiology. Heart and circulatory physiology, Vol. 303, Issue 2, pp. H168-77, 2012

Images

Product Details KIT Ligand ELISA Kit Target details Application Details Handling ProductDetails: References for KIT Ligand Kit (ABIN2748629) back to top
Supplier Images
ELISA image for KIT Ligand ELISA Kit (KITLG) (ABIN2748629) KIT Ligand (KITLG) ELISA Kit