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Human MME ELISA Kit

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Antigen
Membrane Metallo-Endopeptidase (MME) ELISA Kits
  • 6030454K05Rik
  • AV378681
  • C85356
  • CAK
  • CALLA
  • CD10
  • CD167
  • DDR
  • EDDR1
  • HGK2
  • MCK10
  • MME
  • Mmel
  • Nep
  • NEP
  • NTRK4
  • PTK3
  • PTK3A
  • RTK6
  • SFE
  • TRKE
Epitope
AA 52-750
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
13
12
8
1
1
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
125-8000 pg/mL
Minimum Detection Limit
125 pg/mL
Application
ELISA
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Catalog No. ABIN1889325
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.31929058 ABIN417259 Sandwich ELISA Serum, Plasma, Cerebrospinal Fluid, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see 1
0.31929058 ABIN2708108 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 125-8000 pg/mL 125 pg/mL Log in to see
0.001220287 ABIN457043 Sandwich ELISA Serum, Plasma, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Log in to see 1
0.001220287 ABIN1114298 Sandwich ELISA Serum, Plasma, Biological Fluids Log in to see
0.001220287 ABIN1571454 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.001220287 ABIN2703366 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine 0.4-100 ng/mL 0.4 ng/mL Log in to see
0.001220287 ABIN2684979 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 125-8000 pg/mL 125 pg/mL Log in to see
0.001220287 ABIN585264 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.001220287 ABIN2537044 Sandwich ELISA Serum, Plasma, Cerebrospinal Fluid, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.001220287 ABIN2542359 Serum, Plasma, Biological Fluids 6-400 pg/mL 6 pg/mL Log in to see
0.001220287 ABIN2093387 Sandwich ELISA Log in to see
0.001220287 ABIN2076494 Sandwich ELISA Log in to see
0.001220287 ABIN2949776 0.156-10 ng/mL 0.156 ng/mL Log in to see

Top referenced MME ELISA Kit for ELISA

Similar MME ELISA Kits

Application / Reactivity Human
ELISA 14 ELISA Kits

General

Antigen Membrane Metallo-Endopeptidase (MME) ELISA Kits
Epitope AA 52-750
Reactivity Human
Kits with alternative reactivity to:
(13), (12), (8), (1), (1), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 125-8000 pg/mL
Minimum Detection Limit 125 pg/mL
Application ELISA
Pubmed 2 references available
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Product Details MME ELISA Kit

Target details Application Details Handling References for MME Kit (ABIN1889325) Images
Purpose For quantitative detection of human CD10 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human CD10
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human CD10 antibod - 1
  • Lyophilized recombinant human CD10 standar - 10ng/tubex2
  • Biotinylated anti- human CD10 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: CHO
Immunogen sequence: Y52-W750

Target details

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Antigen
Alternative Name MME (MME ELISA Kit Abstract)
Background CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), Neprilysin, or common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.
Gene ID 4311
UniProt P08473
Pathways RTK Signaling

Application Details

Product Details MME ELISA Kit Target details Handling References for MME Kit (ABIN1889325) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human CD10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD10 has been precoated onto 96-well plates. Standards(CHO, Y52-W750) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD10 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human CD10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD10 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 1265, Standard deviation: 48.07, CV(%): 3.8
  • Sample 2: n=16, Mean(pg/ml): 3210, Standard deviation: 179.8, CV(%): 5.6
  • Sample 3: n=16, Mean(pg/ml): 6840, Standard deviation: 321.5, CV(%): 4.7,
  • Sample 1: n=24, Mean(pg/ml): 1526, Standard deviation: 83.93, CV(%): 5.5
  • Sample 2: n=24, Mean(pg/ml): 2761, Standard deviation: 201, CV(%): 7.3
  • Sample 3: n=24, Mean(pg/ml): 6397, Standard deviation: 369.6, CV(%): 6.2
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for MME Kit (ABIN1889325)

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Background publications

Barker, Shipp, DAdamio, Masteller, Reinherz: "The common acute lymphoblastic leukemia antigen gene maps to chromosomal region 3 (q21-q27)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 1, pp. 283-7, 1989

Stephenson, Kenny: "The hydrolysis of alpha-human atrial natriuretic peptide by pig kidney microvillar membranes is initiated by endopeptidase-24.11." in: The Biochemical journal, Vol. 243, Issue 1, pp. 183-7, 1987

Images

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Supplier Images
ELISA image for MME ELISA Kit (Membrane Metallo-Endopeptidase) (ABIN1889325) Human CD10/Neprilysin PicoKine ELISA Kit standard curve