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MME ELISA Kit

MME Reactivity: Human Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Catalog No. ABIN6720026
  • Target See all MME ELISA Kits
    MME (Membrane Metallo-Endopeptidase (MME))
    Reactivity
    • 4
    • 3
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    125 pg/mL - 8000 pg/mL
    Minimum Detection Limit
    125 pg/mL
    Application
    ELISA
    Purpose
    Sandwich ELISA for Quantitative Detection of Antigen
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Characteristics

    Synonyms: Atriopeptidase, CALLA, CD10, CD10 antigen, Common acute lymphocytic leukemia antigen, Enkephalinase, EPN, gp100, Membrane metallo endopeptidase, Membrane metallo endopeptidase variant 1, Membrane metallo endopeptidase variant 2, Membrane metalloendopeptidase, Membrane metalloendopeptidase neutral endopeptidase enkephalinase, Membrane metalloendopeptidase variant 1, Membrane metalloendopeptidase variant 2, NEP, NEP_HUMAN, Neprilysin, Neutral endopeptidase, SFE, Skin fibroblast elastase

    Background: CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), Neprilysin, or common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

    Gene Name: MME

    Production: Natural and recombinant human CD10. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: CHO, Immunogen sequence: Y52-W750

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  • Application Notes

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human CD10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CD10 standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 125pg/mL-8000pg/mL

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target See all MME ELISA Kits
    MME (Membrane Metallo-Endopeptidase (MME))
    Alternative Name
    CD10 - Neprilysin (MME Products)
    Synonyms
    CALLA ELISA Kit, CD10 ELISA Kit, NEP ELISA Kit, SFE ELISA Kit, 6030454K05Rik ELISA Kit, C85356 ELISA Kit, Nep ELISA Kit, neprilysin ELISA Kit, membrane metalloendopeptidase ELISA Kit, membrane metallo endopeptidase ELISA Kit, membrane metallo-endopeptidase ELISA Kit, MME ELISA Kit, Mme ELISA Kit
    Target Type
    Chemical
    Gene ID
    4311
    NCBI Accession
    NP_000893
    UniProt
    P08473
    Pathways
    RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration
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