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Human Neurotrophin 4 ELISA Kit

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Antigen
Neurotrophin 4 (NTF4) ELISA Kits
  • 2900040K06Rik
  • AI462899
  • GLC1O
  • GLC10
  • NT-4
  • NT-4/5
  • NT-5
  • NT4
  • NT4/5
  • NT4P
  • NT5
  • Ntf-5
  • Ntf4
  • NTF5
  • Ntf5
Epitope
AA 81-210
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
18
14
13
5
4
4
4
3
3
2
2
2
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
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Catalog No. ABIN1706065
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN414911 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32693866 ABIN1116176 Sandwich ELISA Serum, Plasma, Biological Fluids 93.75-6000 pg/mL 93.75 pg/mL Log in to see
0.32693866 ABIN2708342 Sandwich ELISA Cell Culture Supernatant, Serum 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN365304 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 0.625-40 ng/mL 0.625 ng/mL Log in to see 2
0.0012117105 ABIN511442 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 250-5000 pg/mL 250 pg/mL Log in to see
0.0012117105 ABIN455988 Serum, Tissue Homogenate, Biological Fluids 93.75-6000 pg/mL 93.75 pg/mL Log in to see
0.0012117105 ABIN771023 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids Log in to see
0.0012117105 ABIN625066 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 3
0.0012117105 ABIN1029391 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012117105 ABIN1112669 Sandwich ELISA Serum, Plasma, Urine, Tissue Samples, Cell Culture Supernatant 800-30000 pg/mL 800 pg/mL Log in to see
0.0012117105 ABIN2685110 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Samples, Urine 800-30000 pg/mL 800 pg/mL Log in to see
0.0012117105 ABIN2685109 Sandwich ELISA Cell Culture Supernatant, Serum 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN2685111 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine Log in to see
0.0012117105 ABIN1847023 Tissue Homogenate, Cell Lysate, Biological Fluids 800-30000 pg/mL 800 pg/mL Log in to see
0.0012117105 ABIN2537192 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.0012117105 ABIN2093673 Sandwich ELISA Log in to see
0.0012117105 ABIN2949883 93.75-6000 pg/mL 93.75 pg/mL Log in to see
0.0012117105 ABIN2116274 Log in to see

Top referenced Neurotrophin 4 ELISA Kit for ELISA

Similar Neurotrophin 4 ELISA Kits

Application / Reactivity Human
ELISA 19 ELISA Kits

General

Antigen Neurotrophin 4 (NTF4) ELISA Kits
Epitope AA 81-210
Reactivity Human
Kits with alternative reactivity to:
(18), (14), (13), (5), (4), (4), (4), (3), (3), (2), (2), (2)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 3 references available
Supplier Log in to see

Product Details Neurotrophin 4 ELISA Kit

Target details Application Details Handling References for Neurotrophin 4 Kit (ABIN1706065) Images
Purpose For quantitative detection of human NT-4 in cell culture supernates and serum.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum
Detection Method Colorimetric
Specificity Natural and recombinant human NT-4
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human NT-4 antibod - 1
  • Lyophilized recombinant human NT-4 standar - 10ng/tubex2
  • Biotinylated anti- human NT-4 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: sf21
Immunogen sequence: G81-A210

Target details

Product Details Neurotrophin 4 ELISA Kit Application Details Handling References for Neurotrophin 4 Kit (ABIN1706065) Images back to top
Antigen
Alternative Name NTF4 (NTF4 ELISA Kit Abstract)
Background Neurotrophin-4(NT-4), also known as neurotrophin-5(NT-5), is a protein that in humans is encoded by the NTF4 gene. Human NT-4 as well as a human NT-4 pseudogene colocalize to chromosome 19 band q13.3. NT-4 is a member of a family of neurotrophic factors, the neurotrophins, that control survival and differentiation of vertebrate neurons(2-4). NT-4 is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase. NT4 plays a physiological role essential for hippocampus- and amygdala-dependent long-term memory and hippocampal long-lasting LTP and that NT4 may be useful in the therapy of acquired disorders of learning and memory.
Gene ID 4909
UniProt P34130
Pathways RTK Signaling

Application Details

Product Details Neurotrophin 4 ELISA Kit Target details Handling References for Neurotrophin 4 Kit (ABIN1706065) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human NT-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NT-4 has been precoated onto 96-well plates. Standards(sf21, G81-A210) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NT-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NT-4 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human NT-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human NT-4 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 248, Standard deviation: 11.16, CV(%): 4.5
  • Sample 2: n=16, Mean(pg/ml): 745, Standard deviation: 38, CV(%): 5.1
  • Sample 3: n=16, Mean(pg/ml): 1243, Standard deviation: 78.31, CV(%): 6.3,
  • Sample 1: n=24, Mean(pg/ml): 280, Standard deviation: 14.84, CV(%): 5.3
  • Sample 2: n=24, Mean(pg/ml): 821, Standard deviation: 46, CV(%): 5.6
  • Sample 3: n=24, Mean(pg/ml): 1402, Standard deviation: 96.74, CV(%): 6.9
Restrictions For Research Use only

Handling

Product Details Neurotrophin 4 ELISA Kit Target details Application Details References for Neurotrophin 4 Kit (ABIN1706065) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Neurotrophin 4 Kit (ABIN1706065)

Product Details Neurotrophin 4 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Sun, Zhang, Li, Wang, Ma, Sun, Liu, Wang, Zhou: "Interaction between neurotrophin 4 and gonadotrophin in bovine oviducts." in: Theriogenology, Vol. 78, Issue 1, pp. 39-48, 2012

Background publications

Xie, Sayah, Chen, Wei, Smith, Liu: "Deficient long-term memory and long-lasting long-term potentiation in mice with a targeted deletion of neurotrophin-4 gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 14, pp. 8116-21, 2000

Ibáñez: "Neurotrophin-4: the odd one out in the neurotrophin family." in: Neurochemical research, Vol. 21, Issue 7, pp. 787-93, 1997

Images

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Supplier Images
ELISA image for Neurotrophin 4 ELISA Kit (NTF4) (ABIN1706065) Human NT-4 PicoKine ELISA Kit standard curve