|Antigen||Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This NFAT1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-NFAT1 AntibodyTarget Details NFAT1 Application Details Handling Images
|Specificity||This antibody is expected to recognize all three reported isoforms of NFATC2 (NP_036472.2, NP_775114.1, NP_001129493.1).|
Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.
|Purification||Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide|
|Immunogen||Peptide with sequence from the internal region of the protein sequence according to NP_036472.2, NP_775114.1, NP_001129493.1. Genename: NFATC2|
Target Details NFAT1Product Details anti-NFAT1 Antibody Application Details Handling Images back to top
|Alternative Name||NFATC2 (NFAT1 Antibody Abstract)|
|Background||Nuclear factor of activated T cells (NFAT) is a family of transcription factors implicated in multiple biological processes including cytokine gene expression, cardiac hypertrophy and adipocyte differentiation. NFAT1 (also known as NFATc2 or NFATp) is a member of this family that is regulated by the calcium-dependent phosphatase calcineurin. When calcineurin is activated by calcium it dephosphorylates multiple residues in the regulatory domain of NFAT1, leading to its translocation to the nucleus and activation of its transcriptional activity. Once in the nucleus, NFAT proteins act synergistically with the AP-1 transcription factor complex to regulate the expression of multiple genes. Serine 54 in mouse NFAT1 has been shown to be important in the regulation of its transcriptional activity.Synonyms: NFAT pre-existing subunit, NFAT-1, NFATC2, NFATP, Nuclear factor of activated T-cells, T-cell transcription factor NFAT1, cytoplasmic 2|
|Pathways||RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-NFAT1 Antibody Target Details NFAT1 Handling Images back to top
Peptide ELISA: 1/64000. Western blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-NFAT1 Antibody Target Details NFAT1 Application Details Images back to top
|Buffer||Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|