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anti-Dog (Canine) NFAT1 antibody for Immunoprecipitation

Recommended NFAT1 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) Antibodies
  • AI607462
  • NF-ATc2
  • NFAT1
  • NFAT1-D
  • NFATC2
  • NFATP
  • Nfatp
Epitope
AA 29-181
33
15
13
7
7
6
4
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human
123
57
50
6
3
2
1
1
1
Host
Mouse
98
17
12
Clonality
Monoclonal
Conjugate
This NFAT1 antibody is un-conjugated
6
6
6
3
3
3
3
3
3
3
3
3
3
3
3
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
71
41
33
20
19
13
9
5
3
2
2
1
1
1
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Catalog No. ABIN968123
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089235755 ABIN968124 IF IP WB Mouse IgG1 AA 29-181 Log in to see 1-NFAT 5

Top referenced anti-NFAT1 antibody for Immunoprecipitation

Similar anti-NFAT1 Antibodies

Application / Reactivity Human Dog (Canine)
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Dot Blot (DB) 19 Antibodies
ELISA 41 Antibodies 2 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 1 Antibodies
Gel Shift (GS) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 7 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 14 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Western Blotting (WB) 69 Antibodies

General

Antigen Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) Antibodies
Epitope AA 29-181
(33), (15), (13), (7), (7), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human
(123), (57), (50), (6), (3), (2), (1), (1), (1)
Host Mouse
(98), (17), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NFAT1 antibody is un-conjugated
(6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(71), (41), (33), (20), (19), (13), (9), (5), (3), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-NFAT1 Antibody

Target Details NFAT1 Application Details Handling References for anti-NFAT1 Antibody (ABIN968123) Images
Cross-Reactivity Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human NFAT-1 aa. 29-181
Clone 1-NFAT
Isotype IgG1

Target Details NFAT1

Product Details anti-NFAT1 Antibody Application Details Handling References for anti-NFAT1 Antibody (ABIN968123) Images back to top
Antigen
Alternative Name NFAT-1 (NFAT1 Antibody Abstract)
Background T cells are activated and induced to proliferate following binding of their respective antigen. The process of includes expression of genes that encode factors (i.e., cytokines) which regulate various cell types. Modulation of gene expression is conducted by an array of specific interactions between transcription factors and DNA. NFAT-1 (Nuclear Factor of Activated T cells) is a transcription factor that regulates expression of the interleukin-2 gene. Thus, NFAT-1 DNA binding activity is undetectable in resting cells, but increases during T-cell activation. NFAT-1, a protein of 921 amino acids, is part of an oligomeric transcription factor that also contains Fra-1 and JunB. NFAT-1 was initially described as a phosphoprotein and is dephosphorylated in activated T cells transformed with the leukemia virus HTLV-l.
Synonyms: NF-ATc2, NFATP, KIAA0611
Molecular Weight 97-135 kDa
Pathways RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFAT1 Antibody Target Details NFAT1 Handling References for anti-NFAT1 Antibody (ABIN968123) Images back to top
Comment

Related Products: ABIN967389, ABIN968537, ABIN968619

Restrictions For Research Use only

Handling

Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details References for anti-NFAT1 Antibody (ABIN968123) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-NFAT1 Antibody (ABIN968123)

Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details Handling Images back to top
Product cited in:

Grader-Beck, van Puijenbroek, Nadler, Boussiotis: "cAMP inhibits both Ras and Rap1 activation in primary human T lymphocytes, but only Ras inhibition correlates with blockade of cell cycle progression." in: Blood, Vol. 101, Issue 3, pp. 998-1006, 2003

Kadereit, Mohammad, Miller, Woods, Listrom, McKinnon, Alali, Bos, Iacobucci, Sramkoski, Jacobberger, Laughlin: "Reduced NFAT1 protein expression in human umbilical cord blood T lymphocytes." in: Blood, Vol. 94, Issue 9, pp. 3101-7, 1999

Good, Maggirwar, Harhaj, Sun: "Constitutive dephosphorylation and activation of a member of the nuclear factor of activated T cells, NF-AT1, in Tax-expressing and type I human T-cell leukemia virus-infected human T cells." in: The Journal of biological chemistry, Vol. 272, Issue 3, pp. 1425-8, 1997

Boise, Petryniak, Mao, June, Wang, Lindsten, Bravo, Kovary, Leiden, Thompson: "The NFAT-1 DNA binding complex in activated T cells contains Fra-1 and JunB." in: Molecular and cellular biology, Vol. 13, Issue 3, pp. 1911-9, 1993

McCaffrey, Luo, Kerppola, Jain, Badalian, Ho, Burgeon, Lane, Lambert, Curran: "Isolation of the cyclosporin-sensitive T cell transcription factor NFATp." in: Science (New York, N.Y.), Vol. 262, Issue 5134, pp. 750-4, 1993

Images

Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details Handling References for anti-NFAT1 Antibody (ABIN968123) back to top
Supplier Images
Western Blotting (WB) image for anti-NFAT1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2) (AA 29-181) (ABIN968123) Western blot analysis of NFAT-1 on a Jurkat cell lysate (Human T-cell leukemia, ATCC ...
Immunofluorescence (IF) image for anti-NFAT1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2) (AA 29-181) (ABIN968123) Immunofluorescence staining of Jurkat cells (Human T-cell leukemia, ATCC TIB-152).