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|Antigen||Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) Antibodies|
|Conjugate||This NFAT1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA)
|3 references available|
|Supplier||Log in to see|
Product Details anti-NFAT1 AntibodyTarget Details NFAT1 Application Details Handling ProductDetails: References for anti-NFAT1 antibody (ABIN358367) Images
|Specificity||This antibody detects NFATC2 pSer330.|
|Purification||Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S330 of human NFATC2.|
Target Details NFAT1Product Details anti-NFAT1 Antibody Application Details Handling ProductDetails: References for anti-NFAT1 antibody (ABIN358367) Images back to top
|Alternative Name||NFATC2 (NFAT1 Antibody Abstract)|
|Background||NFATC2 is a member of the nuclear factor of activated T cells (NFAT) family. It is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response.Synonyms: NFAT pre-existing subunit, NFAT-1, NFATC2, NFATP, Nuclear factor of activated T-cells, T-cell transcription factor NFAT1, cytoplasmic 2|
|Molecular Weight||99654 Da|
|Gene ID||4773, 9606|
|Pathways||RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-NFAT1 Antibody Target Details NFAT1 Handling ProductDetails: References for anti-NFAT1 antibody (ABIN358367) Images back to top
ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-NFAT1 Antibody Target Details NFAT1 Application Details ProductDetails: References for anti-NFAT1 antibody (ABIN358367) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
ProductDetails: References for anti-NFAT1 antibody (ABIN358367)Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details Handling Images back to top
Golks, Tran, Goetschy, Guerini: "Requirement for O-linked N-acetylglucosaminyltransferase in lymphocytes activation." in: The EMBO journal, Vol. 26, Issue 20, pp. 4368-79, 2007
Dong, Patino-Lopez, Candotti, Shaw: "Structure-function analysis of the WIP role in T cell receptor-stimulated NFAT activation: evidence that WIP-WASP dissociation is not required and that the WIP NH2 terminus is inhibitory." in: The Journal of biological chemistry, Vol. 282, Issue 41, pp. 30303-10, 2007
Gibson, Hedgcock, Aufiero, Wilson, Hafner, Tsokos, Wong: "Induction of the CTLA-4 gene in human lymphocytes is dependent on NFAT binding the proximal promoter." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 6, pp. 3831-40, 2007
ImagesProduct Details anti-NFAT1 Antibody Target Details NFAT1 Application Details Handling ProductDetails: References for anti-NFAT1 antibody (ABIN358367) back to top