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anti-Human NFAT1 antibody for Immunocytochemistry

Recommended NFAT1 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) Antibodies
  • AI607462
  • NF-ATc2
  • NFAT1
  • NFAT1-D
  • NFATC2
  • NFATP
  • Nfatp
Epitope
AA 51-69
33
15
14
13
7
7
6
4
4
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
141
70
63
7
3
2
1
1
1
Host
Mouse
101
26
18
Clonality
Monoclonal
Conjugate
This NFAT1 antibody is un-conjugated
6
6
6
4
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
Application
Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
88
46
36
33
29
23
20
19
17
13
2
2
1
1
1
Supplier
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Catalog No. ABIN152663
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010086668 ABIN2745548 ICC IP IHC WB Rabbit full length Log in to see Polyclonal
0.0010086668 ABIN4339297 GS ICC IF IHC IHC (p) IP WB DyLight 350 Mouse IgG1 AA 51-69 Log in to see 25A10-D6-D2
0.0010086668 ABIN4339298 GS ICC IF IHC IHC (p) IP WB DyLight 405 Mouse IgG1 AA 51-69 Log in to see 25A10-D6-D2
0.0010086668 ABIN4339299 GS ICC IF IHC IHC (p) IP WB PE Mouse IgG1 AA 51-69 Log in to see 25A10-D6-D2
0.0010086668 ABIN4339300 GS ICC IF IHC IHC (p) IP WB PerCP Mouse IgG1 AA 51-69 Log in to see 25A10-D6-D2
0.0010086668 ABIN4339301 GS ICC IF IHC IHC (p) IP WB Allophycocyanin Mouse IgG1 AA 51-69 Log in to see 25A10-D6-D2
0.0010086668 ABIN4339302 GS ICC IF IHC IHC (p) IP WB DyLight 755 Mouse IgG1 AA 51-69 Log in to see 25A10-D6-D2
0.0010086668 ABIN4339303 GS ICC IF IHC IHC (p) IP WB DyLight 488 Mouse IgG1 AA 51-69 Log in to see 25A10-D6-D2
0.0010086668 ABIN4339304 GS ICC IF IHC IHC (p) IP WB DyLight 405LS Mouse IgG1 AA 51-69 Log in to see 25A10-D6-D2
0.0010086668 ABIN4339305 GS ICC IF IHC IHC (p) IP WB DyLight 680 Mouse IgG1 AA 51-69 Log in to see 25A10-D6-D2
0.0010086668 ABIN4339306 ELISA GS ICC IF IHC IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 AA 51-69 Log in to see 25A10-D6-D2
0.0010086668 ABIN4339307 ELISA GS ICC IF IHC IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 AA 51-69 Log in to see 25A10-D6-D2
0.0010086668 ABIN4339308 ELISA GS ICC IF IHC IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 AA 51-69 Log in to see 25A10-D6-D2
0.0010086668 ABIN4339309 ELISA GS ICC IF IHC IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 AA 51-69 Log in to see 25A10-D6-D2
0.0010086668 ABIN2963578 ICC IP IHC WB Rabbit Log in to see Polyclonal
0.0010086668 ABIN1869443 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
0.0010086668 ABIN4339295 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal

Top referenced anti-NFAT1 antibody for Immunocytochemistry

  • Human Monoclonal NFAT1 Primary Antibody for GS, ICC - ABIN152663 (2 Publications): Bonnet, Rochefort, Sutendra, Archer, Haromy, Webster, Hashimoto, Bonnet, Michelakis: The nuclear factor of activated T cells in pulmonary arterial hypertension can be therapeutically targeted. in Proceedings of the National Academy of Sciences of the United States of America 2007 (PubMed)

Similar anti-NFAT1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 8 Antibodies 9 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Gel Shift (GS) 14 Antibodies 14 Antibodies
Immunocytochemistry (ICC) 14 Antibodies 14 Antibodies
Immunofluorescence (IF) 14 Antibodies 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies 33 Antibodies
Immunohistochemistry (IHC) 17 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Western Blotting (WB) 33 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) Antibodies
Epitope AA 51-69
(33), (15), (14), (13), (7), (7), (6), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(141), (70), (63), (7), (3), (2), (1), (1), (1)
Host Mouse
(101), (26), (18)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NFAT1 antibody is un-conjugated
(6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(88), (46), (36), (33), (29), (23), (20), (19), (17), (13), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-NFAT1 Antibody

Target Details NFAT1 Application Details Handling References for anti-NFAT1 Antibody (ABIN152663) Images
Specificity Detects nuclear factor of activated T-cells (NFAT). This does not cross react with NFAT2 (NFATc, NFATc1).
Cross-Reactivity (Details) The sequence of this N-terminal peptide is approx. 70% homologous with human NFAT1.
Purification IgG purified
Immunogen A synthetic peptide with sequence A(51)ISSPSGLAYPDDVLDYGL(69) representing amino acid residues 51-69 of mouse NFAT1-A, B and C isoforms.
Clone 25A10-D6-D2
Isotype IgG1

Target Details NFAT1

Product Details anti-NFAT1 Antibody Application Details Handling References for anti-NFAT1 Antibody (ABIN152663) Images back to top
Antigen
Alternative Name NFATC2/NFAT1 (NFAT1 Antibody Abstract)
Background The nuclear factor of activated T-cells (NFAT) transcription complex is required for theexpression of a group of proteins that collectively regulate the immune response. FourNFAT proteins, encoded on separate genes and expressed as several splice variants,have been described: NFAT1, NFAT2, NFAT3, and NFAT4. These proteins show a lowlevel of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors.Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacksmany of the Fos/Jun contact sites observed in its predecessors and its subcellularlocalization is not calcineurin-dependent. Alternate Names: Anti-NFATc antibody, anti-NFATc1 antibody, anti-NFATc2 antibody, anti-NFATc3antibody, anti-NFATp antibody, anti-NFATx antibody.
Gene Symbol: NFATC2
Gene ID 4773, 18019, 311658
Pathways RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFAT1 Antibody Target Details NFAT1 Handling References for anti-NFAT1 Antibody (ABIN152663) Images back to top
Application Notes By WB this antibody detects an approx. 140 kDa protein representing phosphorylated NFAT1 in resting immune cells, and an approx. 120 kDa protein in stimulated cells that represents fully-dephosphorylated NFAT1. This antibody may be useful in Chromatin Immunoprecipitation (PMID: 15347678).
Recommended dilutions: Gel Super Shift Assays 1:1-1:100, Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:1000, Immunoprecipitation 1:10-1:500, Sandwich ELISA, Western Blot 1 µg/mL
Restrictions For Research Use only

Handling

Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details References for anti-NFAT1 Antibody (ABIN152663) Images back to top
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

References for anti-NFAT1 Antibody (ABIN152663)

Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details Handling Images back to top
Product cited in:

Bonnet, Rochefort, Sutendra, Archer, Haromy, Webster, Hashimoto, Bonnet, Michelakis: "The nuclear factor of activated T cells in pulmonary arterial hypertension can be therapeutically targeted." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 27, pp. 11418-23, 2007

Background publications

Kornau, Schenker, Kennedy, Seeburg: "Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95." in: Science (New York, N.Y.), Vol. 269, Issue 5231, pp. 1737-40, 1995