anti-Mouse (Murine) NFAT1 antibody for Enzyme Immunoassay

Recommended NFAT1 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) Antibodies
  • NFATC2
  • AI607462
  • NF-ATc2
  • NFAT1
  • NFAT1-D
  • Nfatp
  • NFATP
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
  • nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
  • NFATC2
  • nfatc2
  • Nfatc2
Epitope
Internal Region
36
15
13
6
6
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
148
84
77
11
8
7
6
6
6
5
1
1
1
Host
Goat
108
26
18
Clonality
Polyclonal
Conjugate
This NFAT1 antibody is un-conjugated
6
6
6
4
4
4
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
90
55
39
34
21
20
19
18
16
10
4
2
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN5551937
$ 375.38
Plus shipping costs $45.00

General

Antigen Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) Antibodies
Epitope Internal Region
(36), (15), (13), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(148), (84), (77), (11), (8), (7), (6), (6), (6), (5), (1), (1), (1)
Host Goat
(108), (26), (18)
Clonality
Polyclonal
Conjugate This NFAT1 antibody is un-conjugated
(6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(90), (55), (39), (34), (21), (20), (19), (18), (16), (10), (4), (2), (2), (1), (1), (1)
Supplier Log in to see

Product Details anti-NFAT1 Antibody

Target Details NFAT1 Application Details Handling Images
Sequence c-QQFRTGLSSP DARYQ
Specificity This antibody is expected to recognize all three reported isoforms of NFATC2 (NP_036472.2, NP_775114.1, NP_001129493.1).
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Immunogen Peptide with sequence from the internal region of the protein sequence according to NP_036472.2, NP_775114.1, NP_001129493.1. Genename: NFATC2

Target Details NFAT1

Product Details anti-NFAT1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name NFATC2 (NFAT1 Antibody Abstract)
Background Nuclear factor of activated T cells (NFAT) is a family of transcription factors implicated in multiple biological processes including cytokine gene expression, cardiac hypertrophy and adipocyte differentiation. NFAT1 (also known as NFATc2 or NFATp) is a member of this family that is regulated by the calcium-dependent phosphatase calcineurin. When calcineurin is activated by calcium it dephosphorylates multiple residues in the regulatory domain of NFAT1, leading to its translocation to the nucleus and activation of its transcriptional activity. Once in the nucleus, NFAT proteins act synergistically with the AP-1 transcription factor complex to regulate the expression of multiple genes. Serine 54 in mouse NFAT1 has been shown to be important in the regulation of its transcriptional activity.Synonyms: NFAT pre-existing subunit, NFAT-1, NFATC2, NFATP, Nuclear factor of activated T-cells, T-cell transcription factor NFAT1, cytoplasmic 2
Gene ID 4773
NCBI Accession NP_036472
UniProt Q13469
Pathways RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFAT1 Antibody Target Details NFAT1 Handling Images back to top
Application Notes Peptide ELISA: 1/64000. Western blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.